Document Detail


Quality control parameters and interpretive criteria for in vitro susceptibility tests with the macrolide azithromycin. Collaborative Antimicrobial Susceptibility Testing Group.
MedLine Citation:
PMID:  2548865     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Quality control parameters for broth microdilution and disk diffusion susceptibility tests were defined and the interpretive criteria for disk diffusion tests reviewed. For interpretation of tests with 15 micrograms azithromycin disks, the following criteria are recommended: greater than or equal to 19 mm for the susceptible category (MIC less than or equal to 2.0 micrograms/ml) and less than or equal to 15 mm for the resistant category (MIC greater than or equal to 8.0 micrograms/ml). Using these criteria, there was 97% overall agreement between broth dilution and disk diffusion tests; Haemophilus influenzae isolates were susceptible to azithromycin by both methods. The quality control strain Staphylococcus aureus ATCC 25923 gave zones of 21 to 26 mm in diameter in a six-laboratory collaborative study. In azithromycin broth microdilution tests the following MIC control limits are recommended: Escherichia coli ATCC 25922, 2.0-8.0 micrograms/ml; Staphylococcus aureus ATCC 29213, 0.25-1.0 micrograms/ml; and Enterococcus faecalis ATCC 29212, 1.0-4.0 micrograms/ml.
Authors:
A L Barry; C Thornsberry; T L Gavan
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology     Volume:  8     ISSN:  0934-9723     ISO Abbreviation:  Eur. J. Clin. Microbiol. Infect. Dis.     Publication Date:  1989 Jun 
Date Detail:
Created Date:  1989-10-06     Completed Date:  1989-10-06     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  8804297     Medline TA:  Eur J Clin Microbiol Infect Dis     Country:  GERMANY, WEST    
Other Details:
Languages:  eng     Pagination:  544-9     Citation Subset:  IM    
Affiliation:
Clinical Microbiology Institute, Tualatin, Oregon 97062.
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MeSH Terms
Descriptor/Qualifier:
Azithromycin
Bacteria / drug effects*,  isolation & purification
Drug Resistance, Microbial
Enterococcus faecalis / drug effects
Erythromycin / analogs & derivatives*,  pharmacology
Escherichia coli / drug effects
Haemophilus influenzae / drug effects
Microbial Sensitivity Tests / standards*
Quality Control
Staphylococcus aureus / drug effects
Statistics as Topic
Chemical
Reg. No./Substance:
114-07-8/Erythromycin; 83905-01-5/Azithromycin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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