| Quality control in flow cytometry for diagnostic pathology. I. Cell surface phenotyping and general laboratory procedures. | |
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MedLine Citation:
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PMID: 1690504 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Flow cytometric analyses have become commonplace in the clinical laboratory for determining cell lineage and for quantitation of cells bearing a given phenotype. Because these assays are being conducted to support diagnoses or assist in determining therapy, it is crucial to ensure that these tests are highly accurate and reproducible within a laboratory and among laboratories involved in similar endeavors. This quality assurance has been slow evolving in clinical flow cytometry for a variety of reasons: the exquisite sensitivity and delicacy of the instrumentation that recognize previously undetectable variations in staining; the constant improvement of the hardware and software; the rapid development of new techniques and reagents of clinical interest; and the failure of any existing specialty or subspecialty to encompass all aspects of flow cytometry. This article provides an overview of quality assurance necessary for the flow cytometric analysis of cell surface markers. Practical experience, published studies, and suggested guidelines from accreditation agencies have been combined to develop the text. |
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Authors:
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J P McCoy; J L Carey; J R Krause |
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Publication Detail:
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Type: Journal Article; Review |
Journal Detail:
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Title: American journal of clinical pathology Volume: 93 ISSN: 0002-9173 ISO Abbreviation: Am. J. Clin. Pathol. Publication Date: 1990 Apr |
Date Detail:
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Created Date: 1990-04-26 Completed Date: 1990-04-26 Revised Date: 2005-11-16 |
Medline Journal Info:
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Nlm Unique ID: 0370470 Medline TA: Am J Clin Pathol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: S27-37 Citation Subset: AIM; IM |
Affiliation:
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Department of Pathology, University of Pittsburgh, Pennsylvania. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Cell Separation Data Interpretation, Statistical Flow Cytometry / instrumentation, standards*, statistics & numerical data Humans Leukocyte Count / instrumentation Pathology, Clinical / standards Phenotype Quality Control Specimen Handling Staining and Labeling |
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