Document Detail

Quality control in the IVF laboratory: in-vitro and in-vivo development of mouse embryos is unaffected by the quality of water used in culture media.
MedLine Citation:
PMID:  2606962     Owner:  NLM     Status:  MEDLINE    
We have examined the effect of media made with tap water or with various purified waters on the fertilization of mouse oocytes, their development to blastocysts, their rate of hatching in vitro and their survival after transfer to recipients. Zona-intact and zona-free embryos, as well as cell clusters from 8-cell stage embryos, were also used. The macromolecular composition of the media was varied. We were unable to find any adverse effect of tap water under any condition examined. The implications of these findings for quality control in IVF units are discussed.
M A George; P R Braude; M H Johnson; D G Sweetnam
Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Human reproduction (Oxford, England)     Volume:  4     ISSN:  0268-1161     ISO Abbreviation:  Hum. Reprod.     Publication Date:  1989 Oct 
Date Detail:
Created Date:  1990-02-09     Completed Date:  1990-02-09     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8701199     Medline TA:  Hum Reprod     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  826-31     Citation Subset:  IM    
Department of Anatomy, University of Cambridge, UK.
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MeSH Terms
Culture Media / analysis
Fertilization in Vitro / methods*
Quality Control
Water Supply*
Reg. No./Substance:
0/Culture Media; 7732-18-5/Water

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