Document Detail


Quality control assessment of influenza and RSV testing in Europe: 2000-01 season.
MedLine Citation:
PMID:  12631989     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The Quality Control Assessment (QCA) was initiated to evaluate the quality of the influenza and respiratory syncytial virus (RSV) testing in the national reference centres belonging to the European Influenza Surveillance Scheme (EISS) network. Samples were coded and sent in two panels of 12 samples within a two week interval to 16 laboratories during the 2000-01 winter season. The antibodies titration by HI test was reported by 60% of the laboratories (n=16), and the results were correct for 56% of them. One false detection of influenza B antibodies was reported by one laboratory, and for the others the sensitivity varied widely. The sensitivity of the tests for the detection of influenza virus varied for A(H3N2) from 10 to 100,000 TCID50/ml. The influenza A subtyping was performed by 87% of the laboratories, and 31% gave correct results. The characterisation of the variants was undertaken by six laboratories and half of them fully achieved it. Fifty six percent of the laboratories used RT-PCR for the diagnosis; the results were specific and the sensitivity equivalent to the cell culture.
Authors:
M Valette; M Aymard
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Publication Detail:
Type:  Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Euro surveillance : bulletin Européen sur les maladies transmissibles = European communicable disease bulletin     Volume:  7     ISSN:  1560-7917     ISO Abbreviation:  Euro Surveill.     Publication Date:  2002 Nov 
Date Detail:
Created Date:  2003-03-12     Completed Date:  2003-04-28     Revised Date:  2013-05-01    
Medline Journal Info:
Nlm Unique ID:  100887452     Medline TA:  Euro Surveill     Country:  France    
Other Details:
Languages:  eng     Pagination:  161-5     Citation Subset:  IM    
Affiliation:
Centre national de Référence Grippe, Laboratoire de Virologie HCL, Lyons, France.
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MeSH Terms
Descriptor/Qualifier:
Antigens, Viral / analysis
Cells, Cultured
Enzyme-Linked Immunosorbent Assay / standards
Europe
False Negative Reactions
Fluorescent Antibody Technique / standards
Humans
Influenza A virus / classification,  isolation & purification*
Influenza B virus / classification,  isolation & purification*
Laboratories / standards*
Population Surveillance
Quality Control
Respiratory Syncytial Viruses / isolation & purification*
Reverse Transcriptase Polymerase Chain Reaction / standards
Sensitivity and Specificity
Chemical
Reg. No./Substance:
0/Antigens, Viral

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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