Document Detail

Quality control of acid phosphatase assay improved by direct reconstitution of lyophilized control materials with citric acid.
MedLine Citation:
PMID:  6640907     Owner:  NLM     Status:  MEDLINE    
Current quality control of assays of acid phosphatase (EC with thymolphthalein substrate is inadequate when certain control-suppliers' instructions are followed for reconstituting and stabilizing human lyophilized control material. If such materials are reconstituted with a citric acid solution, then stored frozen, control vial-to-vial variability is minimized, as are losses of activity in freshly reconstituted controls and losses during room-temperature storage of the control material. Effects of pH of the reconstituting solution on the acid phosphatase activity of human lyophilized controls are also discussed.
K E Blick; M J Commens; R B Passey
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Clinical chemistry     Volume:  29     ISSN:  0009-9147     ISO Abbreviation:  Clin. Chem.     Publication Date:  1983 Dec 
Date Detail:
Created Date:  1984-01-27     Completed Date:  1984-01-27     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  9421549     Medline TA:  Clin Chem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  2093-6     Citation Subset:  IM    
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MeSH Terms
Acid Phosphatase / standards*
Citric Acid
Freeze Drying*
Hydrogen-Ion Concentration
Quality Control
Statistics as Topic
Reg. No./Substance:
0/Citrates; 77-92-9/Citric Acid; EC Phosphatase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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