Document Detail


Quality control of acid phosphatase assay improved by direct reconstitution of lyophilized control materials with citric acid.
MedLine Citation:
PMID:  6640907     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Current quality control of assays of acid phosphatase (EC 3.1.3.2) with thymolphthalein substrate is inadequate when certain control-suppliers' instructions are followed for reconstituting and stabilizing human lyophilized control material. If such materials are reconstituted with a citric acid solution, then stored frozen, control vial-to-vial variability is minimized, as are losses of activity in freshly reconstituted controls and losses during room-temperature storage of the control material. Effects of pH of the reconstituting solution on the acid phosphatase activity of human lyophilized controls are also discussed.
Authors:
K E Blick; M J Commens; R B Passey
Related Documents :
1743997 - Catalytic histochemistry of acid and neutral hydrolases in plant seedlings.
8645707 - Evidence for a glycosylinositolphospholipid-anchored alkaline phosphatase in the aquati...
6342677 - Purification, characterization and immunocytochemical localization of mouse kidney carn...
11838317 - Characterizations of recombinant human tartrate-resistant acid phosphatase from osteosa...
15070857 - Lactose derivatives are inhibitors of trypanosoma cruzi trans-sialidase activity toward...
20041447 - Lc-ms identification of derivatized free fatty acids from adipocere in soil samples.
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Clinical chemistry     Volume:  29     ISSN:  0009-9147     ISO Abbreviation:  Clin. Chem.     Publication Date:  1983 Dec 
Date Detail:
Created Date:  1984-01-27     Completed Date:  1984-01-27     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  9421549     Medline TA:  Clin Chem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  2093-6     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Acid Phosphatase / standards*
Autoanalysis
Citrates*
Citric Acid
Freeze Drying*
Humans
Hydrogen-Ion Concentration
Quality Control
Statistics as Topic
Chemical
Reg. No./Substance:
0/Citrates; 77-92-9/Citric Acid; EC 3.1.3.2/Acid Phosphatase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Discrepancies in measurement of aspartate aminotransferase by continuous-flow analysis.
Next Document:  Accuracy of lactate dehydrogenase isoenzyme estimations by a thin-layer agarose fluorescent techniqu...