| Quality assurance for sperm concentration using latex beads. | |
| | |
MedLine Citation:
|
PMID: 8405529 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
OBJECTIVE: To provide a simple, universally applicable method of quality assurance for sperm counting, thereby reducing intercounting chamber variation. DESIGN: By using a known concentration of latex beads, the sperm:bead ratio can be used to calculate the actual sperm count. MAIN OUTCOME MEASURES: The mean sperm and bead counts were determined in both a Spot-lite hemocytometer (Baxter Diagnostics, McGaw Park, IL) and a Makler chamber (Polymedco Inc., Yorktown, NY) from 21 different ejaculates mixed with a known concentration of beads. The hemocytometer chamber was used as the standard counting chamber because it consistently yielded a low variation in sperm count. The adjusted sperm concentration of the Makler chamber was calculated using the following formula [hemocytometer beads]/[Makler beads] x [Makler sperm]. RESULTS: Observed mean +/- SD sperm counts were significantly different between the hemocytometer chamber (110.6 +/- 66.2 x 10(6)/mL) and Makler chamber (173.3 +/- 103.5 x 10(6)/mL). However, calculated Makler chamber sperm counts (118.1 +/- 76.1 x 10(6)/mL) was not statistically different from observed hemocytometer sperm counts. CONCLUSION: This novel approach to sperm counting using a known concentration of latex beads as a reference material can be used to reduce variation in sperm counting between observers, counting chambers, and possibly computerized sperm analyzers. |
| | |
Authors:
|
A J Peters; L J Zaneveld; R S Jeyendran |
Related Documents
:
|
20056269 - Microencapsulation of canine sperm and its preservation at 4 degrees c. 19298729 - Motile sperm organelle morphology examination is stricter than tygerberg criteria. 16689889 - The effects of cryopreservation on the morphometric dimensions of iberian red deer (cer... 10028159 - Canine silica urolithiasis. risk factors, detection, treatment, and prevention. 17110399 - Testis/sperm-specific histone 2b in the sperm of donors and subfertile patients: variab... 10732059 - Computer-assisted sperm head morphometry analysis (asma) of cryopreserved ram spermatozoa. 18817269 - Alterations of domains in the plasmatic membrane due to damages of the perinuclear thec... 17555859 - A review of the current literature on aetiology and measurement methods of halitosis. 20138489 - Dentin abnormalities in cheek teeth of wild red deer and roe deer from a fluoride-pollu... |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: Fertility and sterility Volume: 60 ISSN: 0015-0282 ISO Abbreviation: Fertil. Steril. Publication Date: 1993 Oct |
Date Detail:
|
Created Date: 1993-11-15 Completed Date: 1993-11-15 Revised Date: 2004-11-17 |
Medline Journal Info:
|
Nlm Unique ID: 0372772 Medline TA: Fertil Steril Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: 702-5 Citation Subset: IM |
Affiliation:
|
Department of Obstetrics and Gynecology, Northwestern University Medical School, Prentice Women's Hospital, Chicago, Illinois. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Equipment and Supplies Humans Latex* Male Microspheres* Quality Assurance, Health Care* Sperm Count / instrumentation, methods* |
| Chemical | |
Reg. No./Substance:
|
0/Latex |
| Comments/Corrections | |
Comment In:
|
Fertil Steril. 1994 Jul;62(1):202-3
[PMID:
8054038
]
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Folinic acid in the treatment of human male infertility.
Next Document: Improvement of post-thaw sperm motility in poor quality human semen.