Document Detail

Quality assurance for polychromatic flow cytometry using a suite of calibration beads.
MedLine Citation:
PMID:  23138348     Owner:  NLM     Status:  MEDLINE    
The quality assurance program presented here provides a means to maximize and maintain the performance of individual flow cytometers in a facility. To optimize performance, we recommend performing all three steps (optimization, calibration and standardization) in this program when a new flow cytometer is installed or whenever the flow cytometer's optical path is altered (e.g., lasers, filters or detectors are replaced). The complete process requires 3-4 h. On a more frequent basis, only a subset of these procedures need to be performed as a part of daily maintenance routines. The data generated can be tracked to monitor the instrument and determine whether service is needed. In addition, the data can provide a metric for whether repairs and upgrades have improved or harmed performance, and for future instrument-to-instrument comparisons. In sum, the procedures presented here represent an updated framework for optimizing, calibrating and standardizing a flow cytometer for daily use.
Stephen P Perfetto; David Ambrozak; Richard Nguyen; Pratip K Chattopadhyay; Mario Roederer
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Publication Detail:
Type:  Journal Article     Date:  2012-11-08
Journal Detail:
Title:  Nature protocols     Volume:  7     ISSN:  1750-2799     ISO Abbreviation:  Nat Protoc     Publication Date:  2012 Dec 
Date Detail:
Created Date:  2012-11-09     Completed Date:  2013-04-16     Revised Date:  2013-10-17    
Medline Journal Info:
Nlm Unique ID:  101284307     Medline TA:  Nat Protoc     Country:  England    
Other Details:
Languages:  eng     Pagination:  2067-79     Citation Subset:  IM    
Flow Cytometry Core Facility, Vaccine Research Center, National Institute of Allergy and Infectious Diseases (NIAID), National Institutes of Health (NIH), Bethesda, MD, USA.
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MeSH Terms
Flow Cytometry / instrumentation*,  methods*
Fluorescent Dyes
Quality Control
Grant Support
Reg. No./Substance:
0/Fluorescent Dyes

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