Document Detail


Quality assurance for polychromatic flow cytometry using a suite of calibration beads.
MedLine Citation:
PMID:  23138348     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The quality assurance program presented here provides a means to maximize and maintain the performance of individual flow cytometers in a facility. To optimize performance, we recommend performing all three steps (optimization, calibration and standardization) in this program when a new flow cytometer is installed or whenever the flow cytometer's optical path is altered (e.g., lasers, filters or detectors are replaced). The complete process requires 3-4 h. On a more frequent basis, only a subset of these procedures need to be performed as a part of daily maintenance routines. The data generated can be tracked to monitor the instrument and determine whether service is needed. In addition, the data can provide a metric for whether repairs and upgrades have improved or harmed performance, and for future instrument-to-instrument comparisons. In sum, the procedures presented here represent an updated framework for optimizing, calibrating and standardizing a flow cytometer for daily use.
Authors:
Stephen P Perfetto; David Ambrozak; Richard Nguyen; Pratip K Chattopadhyay; Mario Roederer
Related Documents :
8887288 - Cardiac output and urea kinetics in dialysis patients: evidence supporting the regional...
23333158 - One poke or two: can intravenous catheters provide an acceptable blood sample? a data s...
24660218 - 3d-printed fluidic devices enable quantitative evaluation of blood components in modifi...
15062938 - Detection of small degree of nonuniformity in dialysate flow in hollow-fiber dialyzer u...
10204878 - Noninvasive determination of coronary blood flow velocity with magnetic resonance imagi...
9158508 - Intra-operative autotransfusion in radical cystectomy.
Publication Detail:
Type:  Journal Article     Date:  2012-11-08
Journal Detail:
Title:  Nature protocols     Volume:  7     ISSN:  1750-2799     ISO Abbreviation:  Nat Protoc     Publication Date:  2012 Dec 
Date Detail:
Created Date:  2012-11-09     Completed Date:  2013-04-16     Revised Date:  2013-10-17    
Medline Journal Info:
Nlm Unique ID:  101284307     Medline TA:  Nat Protoc     Country:  England    
Other Details:
Languages:  eng     Pagination:  2067-79     Citation Subset:  IM    
Affiliation:
Flow Cytometry Core Facility, Vaccine Research Center, National Institute of Allergy and Infectious Diseases (NIAID), National Institutes of Health (NIH), Bethesda, MD, USA. sperfetto@mail.nih.gov
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Calibration
Flow Cytometry / instrumentation*,  methods*
Fluorescent Dyes
Quality Control
Grant Support
ID/Acronym/Agency:
Z99 AI999999/AI/NIAID NIH HHS
Chemical
Reg. No./Substance:
0/Fluorescent Dyes

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Mumps Hoshino and Torii vaccine strains were distinguished from circulating wild strains.
Next Document:  Generation of human induced pluripotent stem cells from urine samples.