Document Detail

Qualitatively distinct patterns of cytokines are released by human dendritic cells in response to different pathogens.
MedLine Citation:
PMID:  16162273     Owner:  NLM     Status:  MEDLINE    
Dendritic cells produce cytokines that regulate the class of the adaptive immune response. Microbial recognition is mediated, at least in part, by pattern recognition receptors such as Toll-like receptors, which influence dendritic cell maturation. In humans it is not yet clear how intact pathogens modulate the developing immune response. To address the effects of intact pathogens on the maturation and effector functions of human dendritic cells, we investigated their responses to a number of microbial pathogens. We studied a range of micro-organisms including Gram-negative bacteria (Escherichia coli and Salmonella enterica sv. typhimurium), Gram-positive cocci (Staphylococcus aureus) and atypical bacteria (Mycobacterium tuberculosis and Mycoplasma hominis) as well as the human protozoal parasite Trichomonas vaginalis. The micro-organisms were fixed in formaldehyde to prevent replication whilst preserving surface morphology. All the pathogens induced similar up-regulation of dendritic cell activation-associated cell surface markers but there was a profound difference in the patterns of cytokines produced by the stimulated dendritic cells. Some pathogens (E. coli, Salmonella enterica sv. typhimurium and S. aureus) induced interleukin-12 (IL-12), IL-10 and interferon-alpha whereas others (M. tuberculosis, Mycoplasma hominis and T. vaginalis) induced only IL-10. This differential effect was not altered by costimulation of the dendritic cells through CD40. These results support the notion that human dendritic cells are plastic in their response to microbial stimuli and that the nature of the pathogen dictates the response of the dendritic cell.
Karen Scott; Maria Manunta; Conrad Germain; Peter Smith; Meleri Jones; Peter Mitchell; Daniele Dessi; Kathleen Branigan Bamford; Robert I Lechler; Pier Luigi Fiori; Graham R Foster; Giovanna Lombardi
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Immunology     Volume:  116     ISSN:  0019-2805     ISO Abbreviation:  Immunology     Publication Date:  2005 Oct 
Date Detail:
Created Date:  2005-09-15     Completed Date:  2005-11-10     Revised Date:  2013-06-07    
Medline Journal Info:
Nlm Unique ID:  0374672     Medline TA:  Immunology     Country:  England    
Other Details:
Languages:  eng     Pagination:  245-54     Citation Subset:  IM    
Department of Immunology, Division of Medicine, Faculty of Medicine, Imperial College at Hammersmith Hospital, London, United Kingdom.
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MeSH Terms
Antigens, Bacterial / immunology*
Antigens, CD40 / immunology
Antigens, Protozoan / immunology*
Cell Differentiation / immunology
Cells, Cultured
Cytokines / biosynthesis*
Dendritic Cells / immunology*
Interferon Type I / biosynthesis
Interleukin-10 / biosynthesis
Interleukin-12 / biosynthesis
Trichomonas vaginalis / immunology
Reg. No./Substance:
0/Antigens, Bacterial; 0/Antigens, CD40; 0/Antigens, Protozoan; 0/Cytokines; 0/Interferon Type I; 0/Ligands; 130068-27-8/Interleukin-10; 187348-17-0/Interleukin-12

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