| Qualitative and quantitative liquid chromatographic analysis methods for the determination of the effects of feed supplements on hen egg yolk phospholipids. | |
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MedLine Citation:
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PMID: 15212482 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Quantitative and qualitative high-performance liquid chromatographic methods were utilized to separate phospholipid classes. After qualitative separation, the fatty acid moieties of each separated phospholipid class were determined using a gas chromatographic method. On the basis of these analyses, the effect of supplemented feeds on hen egg yolk lipids can be evaluated. The supplemented feeds contained 1-5% of vegetable-based or fish oils. The phospholipid content and composition were the same in all feeding groups, the proportions of phosphatidylcholines, phosphatidylethanolamines, and sphingomyelins being 70, 28, and 3%, respectively. In each feeding group, the fatty acid profiles of phosphatidylcholines and sphingomyelins were similar to each other and different from that of phosphatidylethanolamines. The supplemented feeds had a statistically significant (p < 0.05) effect on the fatty acid composition of phosphatidylcholines. The supplements decreased the proportion of saturated fatty acids in total fat, but this effect was not found in phospholipids. |
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Authors:
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Hanna Kivini; Eila P Järvenpää; Heikki Aro; Rainer Huopalahti; Eeva-Liisa Ryhänen |
Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Journal of agricultural and food chemistry Volume: 52 ISSN: 0021-8561 ISO Abbreviation: J. Agric. Food Chem. Publication Date: 2004 Jun |
Date Detail:
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Created Date: 2004-06-23 Completed Date: 2004-08-03 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0374755 Medline TA: J Agric Food Chem Country: United States |
Other Details:
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Languages: eng Pagination: 4289-95 Citation Subset: IM |
Affiliation:
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Department Biochemistry and Food Chemistry, University of Turku, FIN-20014 Turku, Finland. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animal Feed* Animals Chickens Chromatography, High Pressure Liquid / methods* Dietary Supplements Egg Yolk / chemistry* Fatty Acids / analysis Phospholipids / analysis*, isolation & purification |
| Chemical | |
Reg. No./Substance:
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0/Fatty Acids; 0/Phospholipids |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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