| Qualitative functional deficiency of affinity-purified lactoferrin from neutrophils of patients with chronic myelogenous leukemia, and lactoferrin/H-ferritin-cell interactions in a patient with lactoferrin-deficiency with normal numbers of circulating leukocytes. | |
| | |
MedLine Citation:
|
PMID: 2043267 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
The iron-binding proteins lactoferrin (LF) and H-ferritin have been implicated in the negative regulation of myelopoiesis in vitro and in vivo. The present studies evaluated the functional activity of affinity-purified LF from polymorphonuclear neutrophils (PMN) of patients with chronic myelogenous leukemia (CML) and LF/H-ferritin-cell interactions in a nonleukemic patient with LF deficiency with normal levels of circulating blood leukocytes. Affinity-purified CML-PMN-LF was found to be qualitatively deficient as a suppressor of the release of colony-stimulating factors from mononuclear blood cells, adding to previous information from our group documenting defective LF-cell interactions in CML. LF was detected by immunoradiometric assay in PMN of the patient with LF deficiency, but at a much lower level than normal. This LF was found, however, to be active as a suppressor molecular against the patient's cells and normal donor cells. Patient cells were as responsive as normal cells to effects of purified milk LF. Decreased LF levels in this patient were associated with increased levels of monocyte H-ferritin inhibitory activity, consistent with the known suppressive effects in vitro of LF on H-ferritin release from monocytes. Patient marrow hematopoietic progenitor cells were as responsive as progenitors from normal donors to suppression by purified H-ferritin and prostaglandin E1. These results are consistent with a role of LF and H-ferritin in the control of myelopoiesis in this patient. |
| | |
Authors:
|
H E Broxmeyer; D C Bicknell; S Cooper; G Sledge; D E Williams; W A McGuire; T D Coates |
Related Documents
:
|
2004017 - The effects of interferon-alpha on the proliferation of cml progenitor cells in vitro a... 12802927 - Chronic myeloid leukemia associated with b-cell chronic lymphocytic leukemia: evidence ... 7981137 - Megakaryopoiesis in chronic myeloproliferative disorders: immunohistochemical evaluatio... 18577747 - Association between imatinib-resistant bcr-abl mutation-negative leukemia and persisten... 3311217 - Cellular interactions and genetic restriction. 8972557 - Castanospermine, an oligosaccharide processing inhibitor, reduces membrane expression o... |
Publication Detail:
|
Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
|
Title: Pathobiology : journal of immunopathology, molecular and cellular biology Volume: 59 ISSN: 1015-2008 ISO Abbreviation: Pathobiology Publication Date: 1991 |
Date Detail:
|
Created Date: 1991-07-17 Completed Date: 1991-07-17 Revised Date: 2007-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 9007504 Medline TA: Pathobiology Country: SWITZERLAND |
Other Details:
|
Languages: eng Pagination: 26-35 Citation Subset: IM |
Affiliation:
|
Department of Medicine (Hematology/Oncology), Indiana University School of Medicine, Indianapolis. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Alprostadil
/
pharmacology Chromatography, Affinity Colony-Forming Units Assay Colony-Stimulating Factors / secretion* Depression, Chemical Dinoprostone / pharmacology Ferritins / pharmacology* Hematopoietic Stem Cells / drug effects Humans Lactoferrin / blood, deficiency*, isolation & purification, pharmacology Leukemia, Myelogenous, Chronic, BCR-ABL Positive / pathology* Leukocyte Count Leukocytes, Mononuclear / drug effects*, secretion Neutrophils / chemistry* Secretory Rate / drug effects |
| Grant Support | |
ID/Acronym/Agency:
|
CA36464/CA/NCI NIH HHS; CA36740/CA/NCI NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/Colony-Stimulating Factors; 0/Lactoferrin; 363-24-6/Dinoprostone; 745-65-3/Alprostadil; 9007-73-2/Ferritins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Comparison of transferrin receptors, iron content and isoferritin profile in normal and malignant hu...
Next Document: Fluorescent laser scanning microscopy of F-actin disruption in human endometrial stromal cells expre...