Document Detail


Qualitative analysis of algal secretions with multiple mass spectrometric platforms.
MedLine Citation:
PMID:  22608776     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Lipid secretions from algae pose a great opportunity for engineering biofueler feedstocks. The lipid exudates could be interesting from a process engineering perspective because lipids could be collected directly from the medium without harvesting and disrupting cells. We here report on the extracellular secretions of algal metabolites from the strain UTEX 2341 (Chlorella minutissima) into the culture medium. No detailed analysis of these lipid secretions has been performed to date. Using multiple mass spectrometric platforms, we observed around 1000 compounds and were able to annotate 50 lipids by means of liquid chromatography coupled to accurate mass quadrupole time-of-flight mass spectrometry (LC-QTOF), direct infusion with positive and negative electrospray ion trap mass spectrometry and gas chromatography coupled to mass spectrometry (GC-MS). These compounds were annotated by tandem mass spectral (MS/MS) database matching and retention time range filtering. We observed a series of triacylglycerols (TG), sulfoquinovosyldiacylglycerols (SQDG), phosphatidylinositols and phosphatidylglycerols, as well as betaine lipids diacylglyceryl-N,N,N-trimethylhomoserines (DGTS).
Authors:
Tobias Kind; John K Meissen; Dawei Yang; Fernando Nocito; Arpana Vaniya; Yu-Shen Cheng; Jean S Vandergheynst; Oliver Fiehn
Related Documents :
3141086 - Liquid-chromatographic determination of fleroxacin in serum and urine.
543876 - A high pressure liquid chromatographic method for the determination of tetracyclines in...
21639466 - Effects of electric field on confined electrolyte in a hexagonal mesoporous silica.
2490546 - Fluorimetric assay of rufloxacin in serum and in pharmaceutical formulations.
10517136 - Affinity nmr.
19154106 - Trapping effects of green and black tea extracts on peroxidation-derived carbonyl subst...
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2012-05-04
Journal Detail:
Title:  Journal of chromatography. A     Volume:  1244     ISSN:  1873-3778     ISO Abbreviation:  J Chromatogr A     Publication Date:  2012 Jun 
Date Detail:
Created Date:  2012-06-05     Completed Date:  2012-08-31     Revised Date:  2013-08-27    
Medline Journal Info:
Nlm Unique ID:  9318488     Medline TA:  J Chromatogr A     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  139-47     Citation Subset:  IM    
Copyright Information:
Copyright © 2012 Elsevier B.V. All rights reserved.
Affiliation:
UC Davis Genome Center-Metabolomics, University of California, Davis, CA 95616, USA. tkind@ucdavis.edu
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Biofuels
Chlorella / chemistry*,  metabolism
Chromatography, Liquid / methods*
Gas Chromatography-Mass Spectrometry / methods*
Hydrophobic and Hydrophilic Interactions
Lipid Metabolism
Lipids / analysis*,  chemistry
Tandem Mass Spectrometry / methods*
Grant Support
ID/Acronym/Agency:
ES013932/ES/NIEHS NIH HHS; R01 DK078328/DK/NIDDK NIH HHS; R01 DK078328/DK/NIDDK NIH HHS; R01 ES013932/ES/NIEHS NIH HHS; RC2 GM092729/GM/NIGMS NIH HHS; RC2 GM092729/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/Biofuels; 0/Lipids
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Optimisation of a sensitive method based on ultrasound-assisted emulsification-microextraction for t...
Next Document:  Supercritical fluid extraction of free amino acids from broccoli leaves.