Document Detail


Qualitative GC-MS assessment of TCP and TAMORF elimination in rats.
MedLine Citation:
PMID:  20338869     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Nerve agents are highly toxic organophosphorus (OP) compounds. They inhibit acetylcholinesterase (AChE), an enzyme that hydrolyses acetycholine (ACh) in the nervous system. Pathophysiological changes caused by OP poisonings are primarily the consequence of surplus ACh on cholinergic receptors and in the central nervous system. Standard treatment of OP poisoning includes combined administration of carbamates, atropine, oximes and anticonvulsants. In order to improve therapy, new compounds have been synthesised and tested. Tenocyclidine (TCP) and its adamantane derivative 1-[2-(2-thienyl)-2-adamantyl] morpholine (TAMORF) have shown interesting properties against soman poisoning. In this study, we developed a qualitative GC-MS method to measure elimination of TCP and TAMORF through rat urine in order to learn more about the mechanisms through which TCP protects an organism from OP poisoning and to determine the duration of this protective effect. GC-MS showed that six hours after treatment with TCP, rat urine contained only its metabolite 1-thienylcyclohexene, while urine of rats treated with TAMORF contained both TAMORF and its metabolites.
Authors:
Maja Jelena Petek; Ana Lucić Vrdoljak; Gordan Mrsić
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Arhiv za higijenu rada i toksikologiju     Volume:  61     ISSN:  0004-1254     ISO Abbreviation:  Arh Hig Rada Toksikol     Publication Date:  2010 Mar 
Date Detail:
Created Date:  2010-03-26     Completed Date:  2010-07-20     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0373100     Medline TA:  Arh Hig Rada Toksikol     Country:  Croatia    
Other Details:
Languages:  eng     Pagination:  61-7     Citation Subset:  IM    
Affiliation:
Forensic Science Centre "Ivan Vucetić", Zagreb, Croatia. mpetek@mup.hr
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MeSH Terms
Descriptor/Qualifier:
Adamantane / analogs & derivatives*,  pharmacokinetics,  urine
Animals
Gas Chromatography-Mass Spectrometry*
Male
Morpholines / pharmacokinetics*,  urine
Organophosphorus Compounds / poisoning
Piperidines / pharmacokinetics*,  urine
Rats
Rats, Wistar
Thiophenes / pharmacokinetics*,  urine
Chemical
Reg. No./Substance:
0/1-(2-(2-thienyl)-2-adamantyl) morpholine; 0/Morpholines; 0/Organophosphorus Compounds; 0/Piperidines; 0/Thiophenes; 0/tenocyclidine; 281-23-2/Adamantane

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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