| Quadrupole magnetic sorting of porcine islets of Langerhans. | |
| | |
MedLine Citation:
|
PMID: 19505179 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Islet transplantation is emerging as a treatment option for selected patients with type 1 diabetes. Inconsistent isolation, purification, and recovery of large numbers of high-quality islets remain substantial impediments to progress in the field. Removing islets as soon as they are liberated from the pancreas during digestion and circumventing the need for density gradient purification is likely to result in substantially increased viable islet yields by minimizing exposure to proteolytic enzymes, reactive oxygen intermediates, and mechanical stress associated with centrifugation. This study capitalized on the hypervascularity of islets compared with acinar tissue to explore their preferential enrichment with magnetic beads to enable immediate separation in a magnetic field utilizing a quadrupole magnetic sorting. The results demonstrate that (1) preferential enrichment of porcine islets is achievable, but homogeneous bead distribution within the pancreas is difficult to achieve with current protocols; (2) greater than 70% of islets in the dissociated pancreatic tissue were recovered by quadrupole magnetic sorting, but their purity was low; and (3) infused islets purified by density gradients and subsequently passed through quadrupole magnetic sorting had similar potency as uninfused islets. These results demonstrate proof of concept and define the steps for implementation of this technology in pig and human islet isolation. |
| | |
Authors:
|
Rustin M Shenkman; Jeffrey J Chalmers; Bernhard J Hering; Nicole Kirchhof; Klearchos K Papas |
Related Documents
:
|
7865939 - Effects of mr exposure on cell proliferation and migration of chick motoneurons. 3613959 - A uhf probe for nmr micro-imaging experiments. 17585969 - Rats avoid high magnetic fields: dependence on an intact vestibular system. 3951559 - Attempts to train goldfish to respond to magnetic field stimuli. 6978129 - The relief mode. new application of the corneal specular microscope. 11551729 - Development of a 1.0 t mr microscope using a nd-fe-b permanent magnet. 12113999 - Proton magnetic resonance spectroscopic imaging in pediatric major depression. 20549189 - Mucoid degeneration of the posterior cruciate ligament: a case report. 7865939 - Effects of mr exposure on cell proliferation and migration of chick motoneurons. |
Publication Detail:
|
Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Tissue engineering. Part C, Methods Volume: 15 ISSN: 1937-3392 ISO Abbreviation: Tissue Eng Part C Methods Publication Date: 2009 Jun |
Date Detail:
|
Created Date: 2009-06-09 Completed Date: 2009-10-09 Revised Date: 2010-09-07 |
Medline Journal Info:
|
Nlm Unique ID: 101466663 Medline TA: Tissue Eng Part C Methods Country: United States |
Other Details:
|
Languages: eng Pagination: 147-56 Citation Subset: IM |
Affiliation:
|
Department of Chemical and Biomolecular Engineering, The Ohio State University, Columbus, Ohio, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Cell Separation / methods* Glucose / pharmacology Insulin / secretion Islets of Langerhans / cytology*, drug effects Magnetics / methods* Microspheres Staining and Labeling Sus scrofa |
| Grant Support | |
ID/Acronym/Agency:
|
R01 DK068717-01/DK/NIDDK NIH HHS; R01 DK068717-02/DK/NIDDK NIH HHS; R01 DK068757-01/DK/NIDDK NIH HHS; R01 DK068757-02/DK/NIDDK NIH HHS; R01DK068717/DK/NIDDK NIH HHS |
| Chemical | |
Reg. No./Substance:
|
11061-68-0/Insulin; 50-99-7/Glucose |
| Comments/Corrections | |
Erratum In:
|
Tissue Eng Part C Methods. 2009 Sep;15(3):529 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Bioabsorbable material-containing phosphorylcholine group-rich surfaces for temporary scaffolding of...
Next Document: Multi-channelled collagen-calcium phosphate scaffolds: their physical properties and human cell resp...