Document Detail


Quadrupole ion-trap mass spectrometry to locate fatty acids on lipid A from Gram-negative bacteria.
MedLine Citation:
PMID:  12137789     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The structure of lipid A released by mild acid hydrolysis from lipopolysaccharide from two strains of Shigella flexneri with different degrees of acylation was characterized using electrospray ionization (ESI) and ion-trap mass spectrometry. The lipid A was analyzed underivatized with ESI in negative-ion mode. With multiple stages of fragmentation (MS(n)), both the degree of acylation and the positions of the fatty acids on the disaccharide backbone could be determined. It was possible to determine the degree of acylation by the MS(n) technique, where in each MS stage the parent ion was an ion where one fatty acid had been eliminated. One way to determine the location of the fatty acids was by identifying cross-ring fragments of the reducing sugar from parent ions containing different numbers of fatty acids. Another was by identifying a possible charge-driven release of fatty acids situated close to a phosphate group. The fatty acids were otherwise eliminated by a charge-remote fragmentation mechanism. The combined data show the usefulness of ion-trap mass spectrometers for this type of analysis.
Authors:
Anders Kussak; Andrej Weintraub
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Analytical biochemistry     Volume:  307     ISSN:  0003-2697     ISO Abbreviation:  Anal. Biochem.     Publication Date:  2002 Aug 
Date Detail:
Created Date:  2002-07-24     Completed Date:  2003-02-10     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0370535     Medline TA:  Anal Biochem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  131-7     Citation Subset:  IM    
Affiliation:
University College of South Stockholm, SE-141 89 Stockholm, Sweden. anders.kussak@kfc.ki.se
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MeSH Terms
Descriptor/Qualifier:
Acylation
Dysentery, Bacillary
Fatty Acids / analysis*,  chemistry
Lipid A / chemistry*,  isolation & purification
Lipopolysaccharides / chemistry
Shigella flexneri / chemistry*
Spectrometry, Mass, Electrospray Ionization*
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization*
Chemical
Reg. No./Substance:
0/Fatty Acids; 0/Lipid A; 0/Lipopolysaccharides

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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