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Quadruplex real-time polymerase chain reaction assay for molecular diagnosis of Y-chromosomal microdeletions.
MedLine Citation:
PMID:  22270555     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
OBJECTIVE: To develop a rapid and reliable method for molecular diagnosis of Y-chromosomal microdeletions. DESIGN: Study of diagnostic accuracy. SETTING: Molecular diagnostics laboratories in three hospitals. PATIENT(S): A total of 701 men with nonobstructive azoospermia or oligozoospermia from three hospitals. INTERVENTION(S): We developed a quadruplex real-time polymerase chain reaction (PCR) assay and evaluated its performance in molecular diagnosis of Y-chromosomal microdeletions. MAIN OUTCOME MEASURE(S): Analytic sensitivity, analytic specificity, clinical sensitivity, and clinical specificity. RESULT(S): The limit of detection of quadruplex real-time PCR assay was 100 pg genomic DNA. The method attained 100% analytic specificity, 100% clinical sensitivity, and 100% clinical specificity. CONCLUSION(S): We have successfully upgraded the diagnostic method published by the European Academy of Andrology and the European Molecular Genetics Quality Network. Our method was validated to be fast, simple, contamination free, of high analytic sensitivity and specificity. Therefore, it is strongly suggested that such quadruplex real-time PCR assay can be readily applied as clinical routine in the near future.
Authors:
Qiwei Guo; Fenghua Lan; Liangpu Xu; Yu Jiang; Li Xiao; Hailong Huang; Yulin Zhou
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-1-21
Journal Detail:
Title:  Fertility and sterility     Volume:  -     ISSN:  1556-5653     ISO Abbreviation:  -     Publication Date:  2012 Jan 
Date Detail:
Created Date:  2012-1-24     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0372772     Medline TA:  Fertil Steril     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2012 American Society for Reproductive Medicine. Published by Elsevier Inc. All rights reserved.
Affiliation:
Molecular Diagnostics Laboratory, Department of Medical Genetics, Prenatal Diagnosis Center, Maternal and Child Health Hospital, Xiamen, People's Republic of China.
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