| QTIPS: a novel method of unsupervised determination of isotopic amino acid distribution in SILAC experiments. | |
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MedLine Citation:
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PMID: 20451407 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Stable incorporation of labeled amino acids in cell culture is a simple approach to label proteins in vivo for mass spectrometric quantification. Full incorporation of isotopically heavy amino acids facilitates accurate quantification of proteins from different cultures, yet analysis methods for determination of incorporation are cumbersome and time-consuming. We present QTIPS, Quantification by Total Identified Peptides for SILAC, a straightforward, accurate method to determine the level of heavy amino acid incorporation throughout a population of peptides detected by mass spectrometry. Using QTIPS, we show that the incorporation of heavy amino acids in baker's yeast is unaffected by the use of prototrophic strains, indicating that auxotrophy is not a requirement for SILAC experiments in this organism. This method has general utility for multiple applications where isotopic labeling is used for quantification in mass spectrometry. |
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Authors:
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David J Dilworth; Ramsey A Saleem; Richard S Rogers; Hamid Mirzaei; John Boyle; John D Aitchison |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural Date: 2010-04-08 |
Journal Detail:
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Title: Journal of the American Society for Mass Spectrometry Volume: 21 ISSN: 1879-1123 ISO Abbreviation: J. Am. Soc. Mass Spectrom. Publication Date: 2010 Aug |
Date Detail:
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Created Date: 2010-08-02 Completed Date: 2010-12-03 Revised Date: 2011-09-26 |
Medline Journal Info:
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Nlm Unique ID: 9010412 Medline TA: J Am Soc Mass Spectrom Country: United States |
Other Details:
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Languages: eng Pagination: 1417-22 Citation Subset: IM |
Copyright Information:
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Copyright 2010 American Society for Mass Spectrometry. Published by Elsevier Inc. All rights reserved. |
Affiliation:
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Institute for Systems Biology, Seattle, Washington 98103, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Amino Acids / chemistry*, metabolism Isotope Labeling / methods* Isotopes / chemistry* Mass Spectrometry Metabolic Networks and Pathways Molecular Sequence Data Peptide Fragments / chemistry* Saccharomyces cerevisiae / chemistry, metabolism |
| Grant Support | |
ID/Acronym/Agency:
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GM075152/GM/NIGMS NIH HHS; GM076547/GM/NIGMS NIH HHS; P50 GM076547-04/GM/NIGMS NIH HHS; R01 GM075152-02/GM/NIGMS NIH HHS; R01 GM075152-02S1/GM/NIGMS NIH HHS; RR022220/RR/NCRR NIH HHS; U54 RR022220-05/RR/NCRR NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Amino Acids; 0/Isotopes; 0/Peptide Fragments |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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