Document Detail

QCM-Based Measurement of Bond Rupture Forces in DNA Double Helices for Complementarity Sensing.
MedLine Citation:
PMID:  24635388     Owner:  NLM     Status:  Publisher    
After fixing the DNA molecule in the form of a double helix on QCM surface, mechanical oscillations at increasing amplitude cause detorsion of the helix. The force that is necessary for detorsion can be determined from the voltage applied to the QCM at the rupture moment. The high sensitivity of this method is due to the fact that measurements are carried out in the frequency region around the QCM resonance, where any (even very weak) distortions of the consistent oscillating system cause noticeable distortions of the amplitude-frequency dependence, and these distortions are used to fix the rupture moment. The measured rupture forces were within 30-40 pN, the sensitivity was 10-7 mol/l. It was demonstrated that the proposed procedure allows one to determine the factors that affect the stability of the DNA double helix. This procedure can be the basis for the development of a new method of rapid DNA analysis. Experiments performed with model DNA samples showed that it is possible to reveal the correspondence between two DNA samples.
Fedor N Dultsev; Eugeny A Kolosovsky; Ivan A Mik; Alexander A Lomzov; Dmitry V Pyshnyi
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2014-3-17
Journal Detail:
Title:  Langmuir : the ACS journal of surfaces and colloids     Volume:  -     ISSN:  1520-5827     ISO Abbreviation:  Langmuir     Publication Date:  2014 Mar 
Date Detail:
Created Date:  2014-3-18     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9882736     Medline TA:  Langmuir     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
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