| Q-FISH analysis of telomere and chromosome instability in the oesophagus with and without squamous cell carcinoma in situ. | |
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MedLine Citation:
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PMID: 20455255 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Chromosomal and genomic instability due to telomere dysfunction is known to play an important role in carcinogenesis. To study telomere dysfunction in the surrounding background epithelium of squamous cell carcinoma in situ (CIS) of the oesophagus, we measured telomere lengths of basal and parabasal cells of epithelia with and without CIS using quantitative fluorescence in situ hybridization (Q-FISH) and our original software, Tissue Telo. Additionally, we assessed histological inflammation and the anaphase bridge index. In non-cancerous epithelium, telomeres in basal cells were significantly longer than those in parabasal cells, whereas CIS showed a homogeneous telomere pattern in the basal and parabasal cells. Telomeres in basal and parabasal cells were significantly shorter in the background with CIS than in epithelium from age-matched normal controls. Significant negative correlation was observed between the normalized telomere : centromere ratio (reflected telomere length) and the anaphase bridge index in non-cancerous epithelia from both normal controls and the CIS background with no histological inflammation. These findings indicate that tissue stem cells may be located among basal cells, and that telomere length distribution in component cell types differs between CIS and non-cancerous epithelium. We have demonstrated conclusively that oesophageal CIS arises from epithelium with short telomeres and chromosomal instability in the absence of histological inflammation. |
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Authors:
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Kaiyo Takubo; Masahiro Fujita; Naotaka Izumiyama; Ken-ichi Nakamura; Naoshi Ishikawa; Steven S Poon; Mutsunori Fujiwara; Motoji Sawabe; Masaaki Matsuura; Heike Grabsch; Tomio Arai; Junko Aida |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: The Journal of pathology Volume: 221 ISSN: 1096-9896 ISO Abbreviation: J. Pathol. Publication Date: 2010 Jun |
Date Detail:
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Created Date: 2010-05-10 Completed Date: 2010-07-28 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0204634 Medline TA: J Pathol Country: England |
Other Details:
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Languages: eng Pagination: 201-9 Citation Subset: IM |
Affiliation:
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Research Team for Geriatric Pathology, Tokyo Metropolitan Institute of Gerontology, 35-2 Sakae-cho, Itabashi-ku, Tokyo 173-0015, Japan. takubo@tmig.or.jp |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Aged Aged, 80 and over Carcinoma in Situ / genetics*, pathology Carcinoma, Squamous Cell / genetics*, pathology Case-Control Studies Chromosomal Instability / genetics* Esophageal Neoplasms / genetics*, pathology Female Humans In Situ Hybridization, Fluorescence / methods Male Middle Aged Telomere / genetics*, pathology Tumor Cells, Cultured |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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