| Pyruvate-enriched cardioplegia suppresses cardiopulmonary bypass-induced myocardial inflammation. | |
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MedLine Citation:
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PMID: 20971256 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: Cardiopulmonary bypass-induced oxidative stress initiates inflammation that can damage the myocardium. This study tested whether cardioplegia enriched with the intermediary metabolite and antioxidant pyruvate dampens postbypass myocardial inflammation. METHODS: Pigs were maintained on cardiopulmonary bypass while their hearts were arrested for 60 minutes with 4:1 blood:crystalloid cardioplegia, in which the crystalloid contained 188 mM glucose ± 24 mM pyruvate. Pigs were weaned from bypass after 30 minutes of whole blood reperfusion and recovered for 4 hours. Glutathione (GSH) and glutathione disulfide (GSSG) were measured in coronary sinus plasma to indirectly monitor myocardial GSH redox state (GSH/GSSG). Left ventricular myocardium was sampled 4 hours after cardiopulmonary bypass for analyses of C-reactive protein, matrix metalloproteinases 2 and 9 and tissue inhibitor of metalloproteinase-2 (TIMP-2), and to assess neutrophil infiltration by histology and myeloperoxidase assay. RESULTS: Coronary sinus GSH/GSSG fell 70% after cardiopulmonary bypass with control cardioplegia, but pyruvate cardioplegia produced a robust increase in coronary sinus GSH/GSSG that persisted for 4 hours after bypass. Myocardial C-reactive protein content increased 5.6-fold after control bypass, and neutrophil infiltration and myeloperoxidase activity also increased, but pyruvate-fortified cardioplegia prevented these inflammatory effects. Control cardioplegia lowered myocardial TIMP-2 content by 59% and increased matrix metalloproteinase-9 activity by 35% versus nonbypass sham values, but pyruvate cardioplegia increased TIMP-2 content ninefold versus control cardioplegia and prevented the increase in matrix metalloproteinase-9. Matrix metalloproteinase-2 was not affected by bypass ± pyruvate. CONCLUSIONS: Pyruvate-enriched cardioplegia dampens cardiopulmonary bypass-induced myocardial inflammation. Increased GSH/GSSG and TIMP-2 may mediate pyruvate's effects. |
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Authors:
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Myoung-Gwi Ryou; Devin C Flaherty; Besim Hoxha; Hunaid Gurji; Jie Sun; Lisa M Hodge; Albert H Olivencia-Yurvati; Robert T Mallet |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: The Annals of thoracic surgery Volume: 90 ISSN: 1552-6259 ISO Abbreviation: Ann. Thorac. Surg. Publication Date: 2010 Nov |
Date Detail:
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Created Date: 2010-10-25 Completed Date: 2010-11-10 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 15030100R Medline TA: Ann Thorac Surg Country: Netherlands |
Other Details:
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Languages: eng Pagination: 1529-35 Citation Subset: AIM; IM |
Copyright Information:
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Copyright © 2010 The Society of Thoracic Surgeons. Published by Elsevier Inc. All rights reserved. |
Affiliation:
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Department of Integrative Physiology, University of North Texas Health Science Center, Fort Worth, Texas 76107-2699, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Antioxidants / pharmacology C-Reactive Protein / analysis Cardiopulmonary Bypass / adverse effects* Female Glutathione / blood Glutathione Disulfide / blood Heart Arrest, Induced* Male Matrix Metalloproteinase 2 / analysis Matrix Metalloproteinase 9 / analysis Myocarditis / etiology, prevention & control* Myocardium / pathology Neutrophil Infiltration Pyruvic Acid / pharmacology* Swine Tissue Inhibitor of Metalloproteinase-2 / analysis |
| Chemical | |
Reg. No./Substance:
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0/Antioxidants; 127-17-3/Pyruvic Acid; 127497-59-0/Tissue Inhibitor of Metalloproteinase-2; 27025-41-8/Glutathione Disulfide; 70-18-8/Glutathione; 9007-41-4/C-Reactive Protein; EC 3.4.24.24/Matrix Metalloproteinase 2; EC 3.4.24.35/Matrix Metalloproteinase 9 |
| Comments/Corrections | |
Comment In:
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Ann Thorac Surg. 2010 Nov;90(5):1535-6
[PMID:
20971257
]
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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