| Pyruvate decarboxylase encoded by the PDC1 gene contributes, at least partially, to the decarboxylation of alpha-ketoisocaproate for isoamyl alcohol formation in Saccharomyces cerevisiae. | |
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MedLine Citation:
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PMID: 16233064 Owner: NLM Status: PubMed-not-MEDLINE |
Abstract/OtherAbstract:
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Isoamyl alcohol is an important flavor component of yeast-fermented alcoholic beverages. To identify the enzyme and gene involved in the decarboxylation of alpha-ketoisocaproate (alpha-KIC) for isoamyl alcohol formation, the enzyme was partially purified and analyzed by mass spectrometry. The pyruvate decarboxylase encoded by the PDC1 gene was considered a likely candidate enzyme. Genetic analysis showed that the activity of alpha-KIC decarboxylase and production of isoamyl alcohol partially decreased in a pdc1 null mutant and increased in a transformant with a multi-copy plasmid carrying the PDC1 gene. These results indicate that pyruvate decarboxylase encoded by the PDC1 gene contributes, at least partially, to the decarboxylation of alpha-KIC for isoamyl alcohol formation. |
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Authors:
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H Yoshimoto; T Fukushige; T Yonezawa; Y Sakai; K Okawa; A Iwamatsu; H Sone; Y Tamai |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Journal of bioscience and bioengineering Volume: 92 ISSN: 1389-1723 ISO Abbreviation: J. Biosci. Bioeng. Publication Date: 2001 |
Date Detail:
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Created Date: 2005-10-19 Completed Date: 2005-11-03 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 100888800 Medline TA: J Biosci Bioeng Country: Japan |
Other Details:
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Languages: eng Pagination: 83-5 Citation Subset: - |
Affiliation:
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Central Laboratories for Key Technology, Kirin Brewery Co. Ltd., 1-13-5 Fukuura, Kanazawa-ku, Yokohama, Kanagawa 236-0004, Japan. hyoshimoto@kirin.co.jp |
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