| Pyruvate but not lactate prevents NADH-induced myoglobin oxidation. | |
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MedLine Citation:
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PMID: 15890622 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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In this work, we investigated the influence of NADH on the redox state of myoglobin and the roles of pyruvate and lactate in this process. NADH increased the autoxidation rate of myoglobin. Both a drop in pH and partial deoxygenation markedly stimulated the autoxidation process and the influence of NADH. A correlation between met-Mb formation rate and NADH oxidation rate was always observed. The increased rate of Mb autoxidation caused by NADH was inhibited by catalase and pyruvate but not by l-lactate. The antioxidant activity versus H2O2 of both pyruvate and lactate was evidenced by chemiluminescence experiments. The antioxidant activity of lactate disappeared completely in the presence of myoglobin or apo-myoglobin, whereas it was only reduced for pyruvate. These results could be of interest in preventing autoxidation of myoglobin that can contribute to ischemia-reperfusion injury during infarction or high-intensity exercise. |
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Authors:
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Robert A Olek; Jedrzej Antosiewicz; Jerzy Popinigis; Rosita Gabbianelli; Donatella Fedeli; Giancarlo Falcioni |
Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Free radical biology & medicine Volume: 38 ISSN: 0891-5849 ISO Abbreviation: Free Radic. Biol. Med. Publication Date: 2005 Jun |
Date Detail:
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Created Date: 2005-05-13 Completed Date: 2005-10-06 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8709159 Medline TA: Free Radic Biol Med Country: United States |
Other Details:
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Languages: eng Pagination: 1484-90 Citation Subset: IM |
Affiliation:
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Department of Bioenergetics, Jedrzej Sniadecki University School of Physical Education, Wiejska 1, 80-336 Gdansk, Poland. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Antioxidants / metabolism, pharmacology Catalase / metabolism Dose-Response Relationship, Drug Free Radicals / metabolism Horses Hydrogen Peroxide / pharmacology Hydrogen-Ion Concentration Kinetics Lactates / metabolism Lactic Acid / chemistry, metabolism* Myocardium / metabolism Myoglobin / chemistry, metabolism* NAD / chemistry, metabolism* Oxidation-Reduction Oxygen / metabolism* Pyruvic Acid / chemistry, metabolism* Reperfusion Injury Time Factors |
| Chemical | |
Reg. No./Substance:
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0/Antioxidants; 0/Free Radicals; 0/Lactates; 0/Myoglobin; 127-17-3/Pyruvic Acid; 50-21-5/Lactic Acid; 53-84-9/NAD; 7722-84-1/Hydrogen Peroxide; 7782-44-7/Oxygen; EC 1.11.1.6/Catalase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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