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Pyruvate-associated acid resistance in bacteria.
MedLine Citation:
PMID:  24795365     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Glucose confers acid resistance to exponentially growing bacteria by repressing formation of the cAMP-CRP complex and consequently activating acid resistance genes. Therefore, in a glucose-rich growth environment, bacteria are capable of resisting acidic stresses due to low levels of cAMP-CRP. Here we reveal a second mechanism for the glucose-conferred acid resistance. We show that glucose induces acid resistance in exponentially growing bacteria through pyruvate, the glycolysis product. Pyruvate and/or the downstream metabolites induce expression of the small noncoding RNA (sncRNA) Spot42 and the sncRNA in turn activates expression of the master regulator of acid resistance, RpoS. In contrast to glucose, pyruvate has little effects on levels of the cAMP-CRP complex and does not require the complex for its effects on acid resistance. Another important difference between glucose and pyruvate is that pyruvate can be produced by bacteria. This means that bacteria have the potential to protect themselves from acidic stresses by controlling glucose-derived generation of pyruvate, pyruvate-acetate efflux or reversion from acetate to pyruvate. We tested this possibility by shutting down the pyruvate-acetate efflux and found that the resulting accumulation of pyruvate elevated acid resistance. Many sugars can be broken into glucose and the subsequent glycolysis generates pyruvate. Therefore, the pyruvate-associated acid resistance is not confined to glucose-grown bacteria but functional in bacteria grown on various sugars.
Authors:
Jianting Wu; Yannan Li; Zhiming Cai; Ye Jin
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2014-5-2
Journal Detail:
Title:  Applied and environmental microbiology     Volume:  -     ISSN:  1098-5336     ISO Abbreviation:  Appl. Environ. Microbiol.     Publication Date:  2014 May 
Date Detail:
Created Date:  2014-5-5     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7605801     Medline TA:  Appl Environ Microbiol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
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