Document Detail


Pyrroloquinoline quinone is a potent neuroprotective nutrient against 6-hydroxydopamine-induced neurotoxicity.
MedLine Citation:
PMID:  17268846     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Pyrroloquinoline quinone (PQQ), which is an essential nutrient, has been shown to act as an antioxidant. Reactive oxygen species (ROS) are thought to be responsible for neurotoxicity caused by the neurotoxin 6-hydroxydopamine (6-OHDA). In this study, we investigated the ability of PQQ to protect against 6-OHDA-induced neurotoxicity using human neuroblastoma SH-SY5Y. When SH-SY5Y cells were exposed to 6-OHDA in the presence of PQQ, PQQ prevented 6-OHDA-induced cell death and DNA fragmentation. Flow cytometry analysis using the ROS-sensitive fluorescence probe, dihydroethidium, revealed that PQQ reduced elevation of 6-OHDA-induced intracellular ROS. In contrast to PQQ, antioxidant vitamins, ascorbic acid and alpha-tocopherol, had no protective effect. Moreover, we showed that PQQ effectively scavenged superoxide, compared to the antioxidant vitamins. Therefore, our results suggest the protective effect of PQQ on 6-OHDA-induced neurotoxicity is involved, at least in part, in its function as a scavenger of ROS, especially superoxide.
Authors:
Hirokazu Hara; Hideaki Hiramatsu; Tetsuo Adachi
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Neurochemical research     Volume:  32     ISSN:  0364-3190     ISO Abbreviation:  Neurochem. Res.     Publication Date:  2007 Mar 
Date Detail:
Created Date:  2007-06-01     Completed Date:  2007-08-01     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7613461     Medline TA:  Neurochem Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  489-95     Citation Subset:  IM    
Affiliation:
Department of Clinical Pharmaceutics, Gifu Pharmaceutical University, 5-6-1 Mitahora-higashi, Gifu 502-8585, Japan. harah@gifu-pu.ac.jp
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MeSH Terms
Descriptor/Qualifier:
Ascorbic Acid / pharmacology
Cell Line, Tumor
Cell Survival / drug effects*
Flow Cytometry
Free Radical Scavengers / pharmacology
Humans
Neuroblastoma
Neuroprotective Agents / pharmacology*
Neurotoxins / toxicity*
Oxidopamine / antagonists & inhibitors*,  toxicity*
PQQ Cofactor / pharmacology*
Superoxides / metabolism
alpha-Tocopherol / pharmacology
Chemical
Reg. No./Substance:
0/Free Radical Scavengers; 0/Neuroprotective Agents; 0/Neurotoxins; 11062-77-4/Superoxides; 1199-18-4/Oxidopamine; 50-81-7/Ascorbic Acid; 59-02-9/alpha-Tocopherol; 72909-34-3/PQQ Cofactor

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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