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Pyrosequencing-Based Molecular Monitoring of the Intestinal Bacterial Colonization in Preterm Infants.
MedLine Citation:
PMID:  21734604     Owner:  NLM     Status:  Publisher    
OBJECTIVES:: To investigate the previously unexplored diversity of neonatal intestinal microbiota and monitor early intestinal colonization patterns in Korean preterm infants using high-throughput pyrosequencing technology combined with 16S rDNA- based molecular methods METHODS:: A total of 46,369 partial 16S rDNA sequences obtained from 30 fecal samples serially taken from 10 very low birth weight preterm infants were analyzed. RESULTS:: A significant proportion of the molecular species (21.9%) was found to be unclassified. The pathogenic or potentially pathogenic molecular species belonging to the classes Gammaproteobacteria and Bacilli were predominant over the entire observation period. Anaerobic or nonpathogenic molecular species belonging to the class Clostridia (except Clostridium difficile) and phyla Bacteroidetes were ubiquitous even within 72 hours after birth. The proportion of these species increased significantly at 1 month of age. The most ubiquitous and abundant major molecular genera common to all postnatal ages were Escherichia, Enterobacter, Enterococcus, Veillonella, Serratia, Staphylococcus, Roseburia, Acinetobacter, Citrobacter, Bacteroides, Faecalibacterium, Blautia and Streptococcus. CONCLUSIONS:: The diversity and dynamic nature of intestinal bacterial colonization in the very low birth weight preterm infants were revealed using pyrosequencing technology. The results of this pilot study may provide a basis to consider when investigating or interpreting the role of intestinal microbiota in certain preterm infant diseases such as necrotizing enterocolitis or systemic infection.
Ju Young Chang; Son Moon Shin; Jongsik Chun; Jae-Hak Lee; Jeong Kee Seo
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-7-4
Journal Detail:
Title:  Journal of pediatric gastroenterology and nutrition     Volume:  -     ISSN:  1536-4801     ISO Abbreviation:  -     Publication Date:  2011 Jul 
Date Detail:
Created Date:  2011-7-7     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8211545     Medline TA:  J Pediatr Gastroenterol Nutr     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
* Department of Pediatrics, Seoul National University College of Medicine, Seoul, Republic of Korea † Seoul Metropolitan Government Boramae Medical Center, Seoul, Republic of Korea ‡ Department of Pediatrics, Kwandong University College of Medicine, Seoul, Republic of Korea § School of Biological Sciences and Institute of Microbiology, Seoul National University, Seoul, Republic of Korea || Interdisciplinary Program in Bioinformatics, Seoul National University, Seoul, Republic of Korea.
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