| Pyrolysis mass spectra, sulfhydryl and tryptophan content of the embryonic nuclei from adult human normal and nuclear-cataractous lenses. | |
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MedLine Citation:
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PMID: 7397244 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Cataractous state-related alterations were studied by Curie-point pyrolysis low voltage mass spectrometry performed on the embryonic nuclei from adult normal and nuclear-cataractous human lenses. It was shown that the relative intensities of ion signals assigned to sulfur dioxide and tentatively, to other sulfur oxidation products correlate with the increase in lens nuclear color. Since these ion signals may represent pyrolysis fragments from methionine sulfoxide, methionine sulfone, cysteic acid and disulfide compounds present in the parent material, it is concluded that progressive oxidation of sulfur compounds appears to take place during nuclear cataractogenesis. Automated pyrolysis mass spectrometry coupled with multivariate analysis of the spectral data by computer turned out to be a rapid method of characterizing sub-milligram samples of lens material. The results were supported by data obtained by conventional determination of sulfhydryl and tryptophan groups. |
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Authors:
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P M van Haard; H J Hoenders; J Wollensak; J Haverkamp |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Biochimica et biophysica acta Volume: 631 ISSN: 0006-3002 ISO Abbreviation: Biochim. Biophys. Acta Publication Date: 1980 Aug |
Date Detail:
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Created Date: 1980-10-27 Completed Date: 1980-10-27 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0217513 Medline TA: Biochim Biophys Acta Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 177-87 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Cataract
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metabolism* Computers Humans Lens, Crystalline / analysis* Mass Spectrometry Pyrroles / metabolism Sulfhydryl Compounds / analysis* Tryptophan / analysis* |
| Chemical | |
Reg. No./Substance:
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0/Pyrroles; 0/Sulfhydryl Compounds; 73-22-3/Tryptophan |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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