| Pyrogallol inhibits the growth of gastric cancer SNU-484 cells via induction of apoptosis. | |
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MedLine Citation:
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PMID: 18636183 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Pyrogallol (PG) is a polyphenol compound and is known to be an O2*- generator. We evaluated the effects of PG on the growth of human gastric cancer SNU-484 cells in relation to the cell cycle and apoptosis. Dose-dependent inhibition of cell growth was observed in SNU-484 cells with an IC50 of approximately 50 microM following treatment with PG for 72 h. DNA flow cytometric analysis indicated that treatment with PG generally did not induce the specific cell cycle phase arrest. Treatment with 50 microM PG induced apoptosis approximately 20%, as evidenced by sub-G1 cells and annexin V-staining cells. Treatment with PG also induced the loss of mitochondrial membrane potential (Delta psi m) in SNU-484 cells. The intracellular reactive oxygen species (ROS) levels including O2*- were significantly increased in PG-treated cells. Furthermore, the depletion of the intracellular glutathione (GSH) content was observed in cells treated with 50 or 80 microM PG. In conclusion, PG inhibited the growth of human gastric cancer SNU-484 cells by inducing cell cycle arrest as well as triggering apoptosis. The changes in ROS and GSH by PG were closely related to apoptosis in SNU-484 cells. |
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Authors:
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Woo Hyun Park; Mee Na Park; Yong Hwan Han; Sang Woo Kim |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: International journal of molecular medicine Volume: 22 ISSN: 1107-3756 ISO Abbreviation: Int. J. Mol. Med. Publication Date: 2008 Aug |
Date Detail:
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Created Date: 2008-07-18 Completed Date: 2008-09-18 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9810955 Medline TA: Int J Mol Med Country: Greece |
Other Details:
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Languages: eng Pagination: 263-8 Citation Subset: IM |
Affiliation:
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Department of Physiology, Medical School, Research Institute of Clinical Medicine, Chonbuk National University, JeonJu, Korea. parkwh71@chonbuk.ac.kr |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Antioxidants / pharmacology* Apoptosis / drug effects* Cell Cycle / drug effects Cell Line, Tumor / drug effects* Glutathione / metabolism Humans Membrane Potentials / drug effects Mitochondria / drug effects, physiology Pyrogallol / pharmacology* Reactive Oxygen Species / metabolism Stomach Neoplasms* |
| Chemical | |
Reg. No./Substance:
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0/Antioxidants; 0/Reactive Oxygen Species; 70-18-8/Glutathione; 87-66-1/Pyrogallol |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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