| Pyrimidine nucleotidases from human erythrocyte possess phosphotransferase activities specific for pyrimidine nucleotides. | |
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MedLine Citation:
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PMID: 9428647 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Two cytoplasmic forms of pyrimidine nucleotidase (PN-I and PN-II) have been purified from human erythrocytes to apparent homogeneity and partially characterized. They preferentially hydrolyse pyrimidine 5'-monophosphates and 3'-monophosphates respectively. PN-I and PN-II operate as interconverting activities, capable of transferring the phosphate from the pyrimidine nucleoside monophosphate donor(s) to various nucleoside acceptors, including important drugs like 3'-azido-3'-deoxy-thymidine (AZT), cytosine-beta-D-arabinofuranoside (AraC) and 5-fluoro-2'-deoxy-uridine (5FdUrd), pyrimidine analogues widely used in chemotherapy. Kinetic analysis showed linear behaviour for both PN-I and PN-II. PN-I phosphotransferase activity revealed higher affinity for oxynucleosides with respect to deoxy-nucleosides, whereas the contrary seems to be true for PN-II. These results show for the first time that soluble pyrimidine nucleotidases are endowed with pyrimidine-specific phosphotransferase activity. |
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Authors:
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A Amici; M Emanuelli; G Magni; N Raffaelli; S Ruggieri |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: FEBS letters Volume: 419 ISSN: 0014-5793 ISO Abbreviation: FEBS Lett. Publication Date: 1997 Dec |
Date Detail:
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Created Date: 1998-01-27 Completed Date: 1998-01-27 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0155157 Medline TA: FEBS Lett Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 263-7 Citation Subset: IM |
Affiliation:
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Istituto di Biochimica, Facoltà di Medicina e Chirurgia, Università di Ancona, Italy. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Erythrocytes
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enzymology* Humans Nucleotidases / metabolism* Phosphotransferases / metabolism* Pyrimidines / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Pyrimidines; 289-95-2/pyrimidine; EC 2.7.-/Phosphotransferases; EC 3.1.3.-/Nucleotidases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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