| Pyrimidine moiety of thiamin is biosynthesized from pyridoxine and histidine in Saccharomyces cerevisiae. | |
| | |
MedLine Citation:
|
PMID: 8528151 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
The isotopes of [6-13C]- and [5'-2H2]pyridoxine were incorporated efficiently into the pyrimidine moiety of thiamin in S. cerevisiae. The mass fragmentation pattern showed that the C-6 and H-5' atoms of pyridoxine were incorporated into the C-6, and H-5' atoms of the pyrimidine, respectively. These findings, along with out previous results, show that the C-2', C-2, N-1, C-6, C-5, and C-5' unit of the pyrimidine are derived from the C-2', C-2, N-1, C-6, C-5 and C-5' unit of pyridoxine, and that pyrimidine is biosynthesized from pyridoxine and histidine. |
| | |
Authors:
|
K Tazuya; C Azumi; K Yamada; H Kumaoka |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Biochemistry and molecular biology international Volume: 36 ISSN: 1039-9712 ISO Abbreviation: Biochem. Mol. Biol. Int. Publication Date: 1995 Jul |
Date Detail:
|
Created Date: 1996-01-29 Completed Date: 1996-01-29 Revised Date: 2006-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 9306673 Medline TA: Biochem Mol Biol Int Country: AUSTRALIA |
Other Details:
|
Languages: eng Pagination: 883-8 Citation Subset: IM |
Affiliation:
|
Faculty of Pharmaceutical Sciences, Mukogawa Women's University, Hyogo, Japan. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Carbon Isotopes Deuterium Gas Chromatography-Mass Spectrometry Histidine / metabolism* Isotope Labeling / methods Pyridoxine / metabolism* Pyrimidines / metabolism* Saccharomyces cerevisiae / metabolism* Thiamine / biosynthesis* |
| Chemical | |
Reg. No./Substance:
|
0/Carbon Isotopes; 0/Pyrimidines; 59-43-8/Thiamine; 65-23-6/Pyridoxine; 71-00-1/Histidine; 7782-39-0/Deuterium |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Identification of a novel ATP-dependent proteolytic activity in mitochondrial intermembrane space.
Next Document: Fluorescence spectroscopic study of the interaction of adenine and nucleotide with trichosanthin.