| Pyrene-perylene as a FRET pair coupled to the N2'-functionality of 2'-amino-LNA. | |
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MedLine Citation:
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PMID: 17920888 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Detection of nucleic acid hybridization via fluorescence resonance energy transfer (FRET) using pyren-1-ylmethyl and perylen-3-ylmethyl N2'-functionalized 2'-amino-LNA nucleosides incorporated into oligonucleotides exhibited a clear distance dependence of the FRET efficiency, ranging from below 10% when the fluorophores were approximately 40A apart to approximately 90% when the fluorophores were in close proximity. |
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Authors:
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Dorthe Lindegaard; Andreas S Madsen; Irina V Astakhova; Andrei D Malakhov; B Ravindra Babu; Vladimir A Korshun; Jesper Wengel |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2007-05-03 |
Journal Detail:
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Title: Bioorganic & medicinal chemistry Volume: 16 ISSN: 1464-3391 ISO Abbreviation: Bioorg. Med. Chem. Publication Date: 2008 Jan |
Date Detail:
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Created Date: 2008-01-14 Completed Date: 2008-01-29 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9413298 Medline TA: Bioorg Med Chem Country: England |
Other Details:
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Languages: eng Pagination: 94-9 Citation Subset: IM |
Affiliation:
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Nucleic Acid Center, Department of Physics and Chemistry, University of Southern Denmark, Campusvej 55, DK-5230 Odense M, Denmark. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Fluorescence Resonance Energy Transfer* Fluorescent Dyes Nucleic Acid Conformation Nucleic Acid Hybridization Oligonucleotide Probes / chemistry* Perylene / chemistry* Pyrenes / chemistry* |
| Chemical | |
Reg. No./Substance:
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0/Fluorescent Dyes; 0/Oligonucleotide Probes; 0/Pyrenes; 129-00-0/pyrene; 198-55-0/Perylene |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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