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Pyocyanin inhibits both nitric oxide-dependent and independent relaxation in porcine coronary arteries.
MedLine Citation:
PMID:  25399964     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
The effects of the Pseudomonas aeruginosa virulence factor pyocyanin (PCN) on contractile function of porcine coronary arteries was investigated in vitro. Artery rings (5 mm) were suspended in organ baths containing Krebs solution for the measurement of isometric tension. The effect of PCN on resting and precontracted coronary arteries was initially investigated with various agents. Arteries were precontracted with prostaglandin F2α or potassium chloride, and endothelium-dependent relaxations were induced by various agents in the presence of PCN. PCN evoked small amplitude dose-dependent contractions in resting porcine coronary arteries. PCN also amplified the contractile response to prostaglandin F2α, but did not alter responses to carbachol. PCN (0.1-10 μM) significantly inhibited endothelium-dependent relaxations evoked by neurokinin A. PCN also inhibited relaxations evoked by diethylamine nitric oxide (nitric oxide donor), forskolin (adenylate cyclase activator), dibuytyryl-cAMP (cAMP analog), 8-bromo-cGMP (cGMP analog) and P1075 (KATP channel activator), but not isoprenaline (β-adrenoceceptor agonist). These results indicate that physiological concentrations of PCN interfere with multiple intracellular processes involved in vascular smooth muscle relaxation, in particular pathways downstream of nitric oxide release. Thus PCN may alter normal vascular function in patients infected with P. aeruginosa. This article is protected by copyright. All rights reserved.
Authors:
Allison Hempenstall; Gary D Grant; Shailendra Anoopkumar-Dukie; Peter J Johnson
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2014-11-16
Journal Detail:
Title:  Clinical and experimental pharmacology & physiology     Volume:  -     ISSN:  1440-1681     ISO Abbreviation:  Clin. Exp. Pharmacol. Physiol.     Publication Date:  2014 Nov 
Date Detail:
Created Date:  2014-11-17     Completed Date:  -     Revised Date:  2014-11-18    
Medline Journal Info:
Nlm Unique ID:  0425076     Medline TA:  Clin Exp Pharmacol Physiol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
This article is protected by copyright. All rights reserved.
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