| A putrescine-inducible pathway comprising PuuE-YneI in which gamma-aminobutyrate is degraded into succinate in Escherichia coli K-12. | |
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MedLine Citation:
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PMID: 20639325 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Gamma-aminobutyrate (GABA) is metabolized to succinic semialdehyde by GABA aminotransferase (GABA-AT), and the succinic semialdehyde is subsequently oxidized to succinate by succinic semialdehyde dehydrogenase (SSADH). In Escherichia coli, there are duplicate GABA-ATs (GabT and PuuE) and duplicate SSADHs (GabD and YneI). While GabT and GabD have been well studied previously, the characterization and expression analysis of PuuE and YneI are yet to be investigated. By analyzing the amino acid profiles in cells of DeltapuuE and/or DeltagabT mutants, this study demonstrated that PuuE plays an important role in GABA metabolism in E. coli cells. The similarity of the amino acid sequences of PuuE and GabT is 67.4%, and it was biochemically demonstrated that the catalytic center of GabT is conserved as an amino acid residue important for the enzymatic activity in PuuE as Lys-247. However, the regulation of expression of PuuE is significantly different from that of GabT. PuuE is induced by the addition of putrescine to the medium and is repressed by succinate and low aeration conditions; in contrast, GabT is almost constitutive. Similarly, YneI is induced by putrescine, while GabD is not. For E. coli, PuuE is important for utilization of putrescine as a sole nitrogen source and both PuuE and YneI are important for utilization of putrescine as a sole carbon source. The results demonstrate that the PuuE-YneI pathway was a putrescine-inducible GABA degradation pathway for utilizing putrescine as a nutrient source. |
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Authors:
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Shin Kurihara; Kenji Kato; Kei Asada; Hidehiko Kumagai; Hideyuki Suzuki |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-07-16 |
Journal Detail:
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Title: Journal of bacteriology Volume: 192 ISSN: 1098-5530 ISO Abbreviation: J. Bacteriol. Publication Date: 2010 Sep |
Date Detail:
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Created Date: 2010-08-27 Completed Date: 2010-09-13 Revised Date: 2011-07-25 |
Medline Journal Info:
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Nlm Unique ID: 2985120R Medline TA: J Bacteriol Country: United States |
Other Details:
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Languages: eng Pagination: 4582-91 Citation Subset: IM |
Affiliation:
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Division of Applied Biology, Graduate School of Science and Technology, Kyoto Institute of Technology, Goshokaido-cho Matsugasaki, Sakyo-ku, Kyoto 606-8585, Japan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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4-Aminobutyrate Transaminase
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genetics,
metabolism Escherichia coli K12 / metabolism* Escherichia coli Proteins / genetics, metabolism* Gene Expression Regulation, Bacterial / drug effects, genetics Models, Biological Putrescine / pharmacology* Signal Transduction / drug effects, genetics Succinate-Semialdehyde Dehydrogenase / genetics, metabolism Succinic Acid / metabolism* gamma-Aminobutyric Acid / metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Escherichia coli Proteins; 110-15-6/Succinic Acid; 110-60-1/Putrescine; 56-12-2/gamma-Aminobutyric Acid; EC 1.2.1.24/Succinate-Semialdehyde Dehydrogenase; EC 2.6.1.19/4-Aminobutyrate Transaminase |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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