| Putative sperm fusion protein IZUMO and the role of N-glycosylation. | |
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MedLine Citation:
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PMID: 18952059 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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IZUMO is the mouse sperm protein proven to be essential for fusion with eggs. It contains one immunoglobulin-like domain with a conserved glycosylation site within. In the present paper, we produced transgenic mouse lines expressing unglycosylated IZUMO (N204Q-IZUMO) in Izumo1 -/- background. The expression of N204Q-IZUMO rescued the infertile phenotype of IZUMO disrupted mice, indicating glycosylation is not essential for fusion-facilitating activity of IZUMO. The N204Q-IZUMO was produced in testis in comparable amounts to wild-type IZUMO, but the amount of N204Q-IZUMO on sperm was significantly decreased by the time sperm reached the cauda epididymis. These data suggest that glycosylation is not essential for the function of IZUMO, but has a role in protecting it from fragmentation in cauda epididymis. |
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Authors:
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Naokazu Inoue; Masahito Ikawa; Masaru Okabe |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2008-10-24 |
Journal Detail:
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Title: Biochemical and biophysical research communications Volume: 377 ISSN: 1090-2104 ISO Abbreviation: Biochem. Biophys. Res. Commun. Publication Date: 2008 Dec |
Date Detail:
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Created Date: 2008-11-25 Completed Date: 2008-12-15 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0372516 Medline TA: Biochem Biophys Res Commun Country: United States |
Other Details:
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Languages: eng Pagination: 910-4 Citation Subset: IM |
Affiliation:
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Research Institute for Microbial Diseases, Osaka University, Yamadaoka 3-1, Suita, Osaka 565-0871, Japan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Animals Cell Fusion Dogs Epididymis / cytology, growth & development, metabolism Female Fertility* / genetics Glycosylation Humans Immunoglobulins / genetics, metabolism* Male Membrane Proteins / genetics, metabolism* Mice Mice, Transgenic Molecular Sequence Data Ovum / metabolism, physiology Rats Spermatozoa / metabolism, physiology* |
| Chemical | |
Reg. No./Substance:
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0/Immunoglobulins; 0/Izumo1 protein, mouse; 0/Membrane Proteins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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