Document Detail

Putative pacemakers of crayfish show clock proteins interlocked with circadian oscillations.
MedLine Citation:
PMID:  20952622     Owner:  NLM     Status:  MEDLINE    
Although the molecular mechanisms that control circadian rhythms in many animals, particularly in the fly, are well known, molecular and biochemical studies addressing the location and function of the proteins and genes contributing to the cycling of the clock in crayfish Procambarus clarkii are scarce. In this study, we investigated whether three proteins that interact in the feedback loop of the molecular clock described for Drosophila are expressed in the putative circadian pacemakers of crayfish retina, eyestalk and brain and whether their expression cycles in a manner consistent with elements of the circadian clock. Here we identified PER, TIM and CLK immunoreactivity in the cytoplasm and nucleus of cells located in the retina as well as in clusters of cells and neuropils of the optic ganglia, lateral protocerebrum and brain. Brain clusters 6, 10, 9 and 11, in particular, showed Per, Tim and Clk-like immunoreactivity at the perikarya and nucleus, and these antigens colocalized at Zeitgeber time (ZT) 0 and/or ZT 12. A biochemical assay demonstrated circadian functionality of Per, Tim and Clk proteins. Both in the eyestalk and in the brain, these proteins demonstrated apparent daily and circadian rhythms. The presence and colocalization of these clock proteins in the cytoplasm and/or nucleus of several cells of retina, optic lobe and brain, depending on time, as well as their circadian oscillations, suggest interactions between positive and negative transcription factors and clock proteins similar to those forming the feedback loop of the canonical model proposed for different animals.
Elsa G Escamilla-Chimal; Rosa María Velázquez-Amado; Tatiana Fiordelisio; María Luisa Fanjul-Moles
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Journal of experimental biology     Volume:  213     ISSN:  1477-9145     ISO Abbreviation:  J. Exp. Biol.     Publication Date:  2010 Nov 
Date Detail:
Created Date:  2010-10-18     Completed Date:  2011-01-27     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0243705     Medline TA:  J Exp Biol     Country:  England    
Other Details:
Languages:  eng     Pagination:  3723-33     Citation Subset:  IM    
Departamento de Ecología y Recursos Naturales, Facultad de Ciencias UNAM, Ciudad Universitaria, México 04510, México.
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MeSH Terms
Antibody Specificity
Astacoidea / cytology,  metabolism*
Biological Clocks*
Blotting, Western
Brain / cytology,  metabolism
CLOCK Proteins / metabolism*
Circadian Rhythm / physiology*
Eye / cytology,  metabolism
Fluorescent Antibody Technique
Reproducibility of Results
Reg. No./Substance:
EC Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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