| Purple sweet potato pigments protect murine thymocytes from ⁶⁰Co γ-ray-induced mitochondria-mediated apoptosis. | |
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MedLine Citation:
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PMID: 20698744 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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PURPOSE: Purple sweet potato (PSP) pigments have been widely accepted as antioxidants but their radioprotective effect still remains unclear. In this study we investigated the effect of PSP pigments on ⁶⁰Co γ-ray-induced mitochondria-mediated apoptosis in murine thymocytes. MATERIALS AND METHODS: The murine thymocytes were pretreated by PSP pigments before exposure to 4 Gy ⁶⁰Co γ-rays. Flow cytometry analysis was used to measure apoptotic cells and mitochondrial membrane potential. Reactive oxygen species (ROS) were detected using 2',7',-dichlorofluorescein diacetate (DCFH-DA) probe and the activity of antioxidant enzymes was tested by biochemical assay after irradiation. Cytochrome c, caspase-3 and poly ADP-ribose polymerase (PARP) were measured by Western blotting. RESULTS: After treatment with PSP pigments and exposure to 4 Gy radiation the apoptosis of thymocytes was reduced and the mitochondrial transmembrane potential was maintained compared to control cells. In the presence of PSP pigments, ROS were reduced and the activities of glutathione peroxidase (GSH-px) and superoxide dismutase (SOD) were protected and in some cases increased. All the pro-apoptotic proteins (cytochrome oxidase, caspase 3 and PARP) decreased in PSP pigments pretreated thymocytes compared to irradiated cells in the absence of PSP pigments. CONCLUSIONS: Pre-treatment with PSP pigments significantly inhibited ⁶⁰Co γ-ray-induced mitochondria-mediated apoptosis. This radioprotective effect might be related to ROS scavenging, the enhancement of the activity of antioxidant enzymes, the maintenance of mitochondrial transmembrane potential, and the sequential inhibition of cytochrome c release and downstream caspase and PARP cleavage. |
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Authors:
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Jing Xie; Yan-Tao Han; Chun-Bo Wang; Wen-Gong Yu |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-08-10 |
Journal Detail:
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Title: International journal of radiation biology Volume: 86 ISSN: 1362-3095 ISO Abbreviation: Int. J. Radiat. Biol. Publication Date: 2010 Dec |
Date Detail:
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Created Date: 2010-12-01 Completed Date: 2011-01-12 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8809243 Medline TA: Int J Radiat Biol Country: England |
Other Details:
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Languages: eng Pagination: 1061-9 Citation Subset: IM; S |
Affiliation:
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Department of Pharmacochemistry, Ocean University of China, Qingdao, PR China. |
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Apoptosis / drug effects, radiation effects Caspase 3 / metabolism Cell Survival / drug effects, radiation effects Cells, Cultured Cobalt Radioisotopes / toxicity Cytochromes c / metabolism Gamma Rays / adverse effects Glutathione Peroxidase / metabolism Ipomoea batatas Membrane Potential, Mitochondrial / drug effects, radiation effects Mice Mitochondria / drug effects, metabolism, radiation effects Pigments, Biological / pharmacology* Poly(ADP-ribose) Polymerases / metabolism Radiation-Protective Agents / pharmacology* Reactive Oxygen Species / metabolism Superoxide Dismutase / metabolism T-Lymphocytes / drug effects*, metabolism, pathology, radiation effects* |
| Chemical | |
Reg. No./Substance:
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0/Cobalt Radioisotopes; 0/Pigments, Biological; 0/Radiation-Protective Agents; 0/Reactive Oxygen Species; 9007-43-6/Cytochromes c; EC 1.11.1.9/Glutathione Peroxidase; EC 1.15.1.1/Superoxide Dismutase; EC 2.4.2.30/Poly(ADP-ribose) Polymerases; EC 3.4.22.-/Casp3 protein, mouse; EC 3.4.22.-/Caspase 3 |
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