Document Detail


Purple membrane vesicles: morphology and proton translocation.
MedLine Citation:
PMID:  17009     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Purple membrane vesicles prepared by different techniques differ widely in their morphology and ability to establish a proton gradient in the light. The procedures used to prepare active vesicles do not completely dissociate the purple membrane and thus preserve a preferential orientation of the protein, while most of the lipid is exchanged for added lipid. Responses to illumination are largely determined by the size of the vesicles and the degree to which bacteriorhodopsin is preferentially oriented. Any attempt to compare the interaction of different lipids with bacteriorhodopsin by measuring the pH response must take these factors into account. With an improved technique we have obtained vesicles of rather uniform size and bacteriorhodopsin orientation, which accumulate protons with an initial rate of 160 ng H+ sec-1 mg-1 protein at light intensities of 10(6) erg cm-2 sec-1. The kinetics of the process are complex and at present insufficiently understood.
Authors:
S B Hwang; W Stoeckenius
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The Journal of membrane biology     Volume:  33     ISSN:  0022-2631     ISO Abbreviation:  J. Membr. Biol.     Publication Date:  1977 May 
Date Detail:
Created Date:  1977-07-18     Completed Date:  1977-07-18     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0211301     Medline TA:  J Membr Biol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  325-50     Citation Subset:  IM; S    
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MeSH Terms
Descriptor/Qualifier:
Bacteriorhodopsins* / isolation & purification,  physiology
Carotenoids* / physiology
Circular Dichroism
Deoxycholic Acid
Freeze Fracturing
Halobacterium / physiology
Hydrogen-Ion Concentration
Lipids / analysis
Microscopy, Electron
Polyethylene Glycols
Protein Conformation
Protons
Spectrophotometry
Chemical
Reg. No./Substance:
0/Lipids; 0/Polyethylene Glycols; 0/Protons; 36-88-4/Carotenoids; 53026-44-1/Bacteriorhodopsins; 83-44-3/Deoxycholic Acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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