| Purple Rice Extract and Anthocyanidins of the Constituents Protect against Light-Induced Retinal Damage in Vitro and in Vivo. | |
| | |
MedLine Citation:
|
PMID: 21175193 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
|
This study evaluated the protective effects of purple rice ( Oryza sativa L.) bran extract (PRE) and its major anthocyanidins (cyanidin and peonidin) against light-induced retinal damage. In an in vitro experiment, cultured murine photoreceptor cells (661W) were damaged by a 24 h exposure to light. Viability of 661W after light treatment, assessed by the tetrazolium salt (WST-8) assay and Hoechst 33342 nuclear staining, was improved by the addition of PRE, cyanidin, and peonidin. Intracellular radical activation in 661W, evaluated using the reactive oxygen species (ROS) sensitive probe 5-(and 6)-chloromethyl-2,7-dichlorodihydrofluorescein diacetate acetyl ester (CM-H(2)DCFDA), was reduced by PRE and its anthocyanidins. Electron spin resonance (ESR) measurements showed that PRE, peonidin, and cyanidin all exhibited radical scavenging activities against 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical, superoxide anion radical ((•)O(2)(-)), and hydroxyl radical ((•)OH). In an in vivo mouse experiment, intravitreous injection of PRE significantly suppressed photoreceptor degeneration induced by exposure to light as revealed by histological analysis using hematoxylin-eosin staining. These findings suggest that PRE and its anthocyanidins possess protective effects with antioxidation mechanism in both in vitro and in vivo models of retinal diseases. |
| | |
Authors:
|
Junji Tanaka; Tomohiro Nakanishi; Kenjirou Ogawa; Kazuhiro Tsuruma; Masamitsu Shimazawa; Hiroshi Shimoda; Hideaki Hara |
Related Documents
:
|
12932083 - Nonphotic phase shifting in female syrian hamsters: interactions with the estrous cycle. 12723883 - Plasticity of hamster circadian entrainment patterns depends on light intensity. 16668423 - Small light-harvesting antenna does not protect from photoinhibition. |
Publication Detail:
|
Type: Journal Article Date: 2010-12-22 |
Journal Detail:
|
Title: Journal of agricultural and food chemistry Volume: 59 ISSN: 1520-5118 ISO Abbreviation: J. Agric. Food Chem. Publication Date: 2011 Jan |
Date Detail:
|
Created Date: 2011-01-19 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 0374755 Medline TA: J Agric Food Chem Country: United States |
Other Details:
|
Languages: eng Pagination: 528-36 Citation Subset: IM |
Affiliation:
|
Molecular Pharmacology, Department of Biofunctional Evaluation, Gifu Pharmaceutical University, 1-25-4 Daigaku-nishi, Gifu 501-1196, Japan. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Topological study of the late steps of the artemisinin decomposition process: modeling the outcome o...
Next Document: Direct, One-Pot Reductive Alkylation of Anilines with Functionalized Acetals Mediated by Triethylsil...