Document Detail

Purity for clarity: the need for purification of tumor cells in DNA microarray studies.
MedLine Citation:
PMID:  15744349     Owner:  NLM     Status:  MEDLINE    
It is now well established that gene expression profiling using DNA microarrays can provide novel information about various types of hematological malignancies, which may lead to identification of novel diagnostic markers. However, to successfully use microarrays for this purpose, the quality and reproducibility of the procedure need to be guaranteed. The quality of microarray analyses may be severely reduced, if variable frequencies of nontarget cells are present in the starting material. To systematically investigate the influence of different types of impurity, we determined gene expression profiles of leukemic samples containing different percentages of nonleukemic leukocytes. Furthermore, we used computer simulations to study the effect of different kinds of impurity as an alternative to conducting hundreds of microarray experiments on samples with various levels of purity. As expected, the percentage of erroneously identified genes rose with the increase of contaminating nontarget cells in the samples. The simulations demonstrated that a tumor load of less than 75% can lead to up to 25% erroneously identified genes. A tumor load of at least 90% leads to identification of at most 5% false-positive genes. We therefore propose that in order to draw well-founded conclusions, the percentage of target cells in microarray experiment samples should be at least 90%.
D de Ridder; C E van der Linden; T Schonewille; W A Dik; M J T Reinders; J J M van Dongen; F J T Staal
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Leukemia     Volume:  19     ISSN:  0887-6924     ISO Abbreviation:  Leukemia     Publication Date:  2005 Apr 
Date Detail:
Created Date:  2005-04-29     Completed Date:  2005-05-26     Revised Date:  2013-03-04    
Medline Journal Info:
Nlm Unique ID:  8704895     Medline TA:  Leukemia     Country:  England    
Other Details:
Languages:  eng     Pagination:  618-27     Citation Subset:  IM    
Department of Immunology, Erasmus MC, University Medical Center Rotterdam, Rotterdam, The Netherlands.
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MeSH Terms
Cell Separation / methods*
Computer Simulation
DNA, Neoplasm / isolation & purification*
Gene Expression Regulation, Leukemic
Guidelines as Topic
Leukemia, Myelogenous, Chronic, BCR-ABL Positive / genetics*
Leukemia-Lymphoma, Adult T-Cell / genetics*
Leukocytes, Mononuclear / cytology
Oligonucleotide Array Sequence Analysis / methods*,  standards
Reproducibility of Results
Reg. No./Substance:
0/DNA, Neoplasm

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