| Purinergic signals regulate daily S-phase cell activity in the ciliary marginal zone of the zebrafish retina. | |
| | |
MedLine Citation:
|
PMID: 21454291 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Regeneration and growth that occur in the adult teleost retina have been helpful in identifying molecular and cellular mechanisms underlying cell proliferation and differentiation. Here, it is reported that S-phase cell number, in the ciliary marginal zone (CMZ) of the adult zebrafish retina, exhibits day-night variations with a mid-light phase peak. Oscillations persist for 24 h in constant darkness (DD), suggesting control by a circadian component. However, variations in the S-phase nuclei number were rapidly dampened and not present during and after a second day in DD. An ADPβS treatment significantly enhanced S-phase activity at night to mid-light levels, as assessed by in vivo BrdU incorporation in a 2-h interval. Moreover, daylight increase in S-phase cell number was completely abolished when extracellular nucleotide levels or their extracellular hydrolysis by ectonucleoside triphosphate diphosphohydrolases (NTPDases) were significantly disrupted or when a selective antagonist of purinergic P2Y1 receptors was intraocularly injected before BrdU exposure. Extracellular nucleotides and NTPDase action were also important for maintaining nocturnal low levels of S-phase activity in the CMZ. Finally, we showed that mRNAs of NTPDases 1, 2 (3 isoforms), and 3 as well as of P2Y1 receptor are present in the neural retina of zebrafish. NTPDase mRNA expression exhibited a 2-fold increment in light versus dark conditions as assessed by quantitative RT-PCR, whereas P2Y1 receptor mRNA levels did not show significant day-night variations. This study demonstrates a key role for nucleotides, principally ADP as a paracrine signal, as well as for NTPDases, the plasma membrane-bound enzymes that control extracellular nucleotide concentration, for inducing S-phase cell entry in the CMZ-normally associated with retinal growth-throughout the light-dark cycle. |
| | |
Authors:
|
Maria Jimena Ricatti; Ariadna Gabriela Battista; Maria Zorrilla Zubilete; Maria Paula Faillace |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Journal of biological rhythms Volume: 26 ISSN: 1552-4531 ISO Abbreviation: J. Biol. Rhythms Publication Date: 2011 Apr |
Date Detail:
|
Created Date: 2011-04-01 Completed Date: 2011-08-04 Revised Date: 2011-08-05 |
Medline Journal Info:
|
Nlm Unique ID: 8700115 Medline TA: J Biol Rhythms Country: United States |
Other Details:
|
Languages: eng Pagination: 107-17 Citation Subset: IM |
Copyright Information:
|
© 2011 Sage Publications |
Affiliation:
|
Facultad de Medicina, Universidad de Buenos Aires, Ciudad de Buenos Aires, Argentina. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Adenosine Diphosphate
/
analogs & derivatives,
metabolism,
pharmacology Adenosine Triphosphate / metabolism Animals Apyrase / pharmacology Cell Differentiation / drug effects Cell Nucleus / metabolism Circadian Clocks / physiology Enzyme Inhibitors / pharmacology Extracellular Space / metabolism Hexokinase / pharmacology Photoperiod Purinergic P2Y Receptor Antagonists / pharmacology Pyrophosphatases / genetics, metabolism RNA, Messenger / genetics, metabolism Receptors, Purinergic P2Y1 / genetics, metabolism* Retina / cytology, enzymology, metabolism* S Phase / drug effects, physiology* Signal Transduction Thionucleotides / pharmacology Zebrafish |
| Chemical | |
Reg. No./Substance:
|
0/Enzyme Inhibitors; 0/Purinergic P2Y Receptor Antagonists; 0/RNA, Messenger; 0/Receptors, Purinergic P2Y1; 0/Thionucleotides; 35094-45-2/adenosine 5'-O-(2-thiodiphosphate); 56-65-5/Adenosine Triphosphate; 58-64-0/Adenosine Diphosphate; EC 2.7.1.1/Hexokinase; EC 3.6.1.-/Pyrophosphatases; EC 3.6.1.5/Apyrase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Gastrin-releasing peptide modulates fast delayed rectifier potassium current in Per1-expressing SCN ...
Next Document: Lab mice in the field: unorthodox daily activity and effects of a dysfunctional circadian clock alle...