Document Detail

Purinergic-induced signaling in C11-MDCK cells inhibits the secretory Na-K-Cl cotransporter.
MedLine Citation:
PMID:  12917108     Owner:  NLM     Status:  MEDLINE    
Purinergic inhibition of Na-K-Cl cotransport has been noted in various renal epithelial cells derived from the collecting tubule, including Madin-Darby canine kidney (MDCK) cells. In recent studies, we have observed purinergic inhibition of Na-K-Cl cotransport in C11-MDCK subclones (alpha-intercalated-like cells). Interestingly, Na-K-Cl cotransport activity was also detected in C7-MDCK subclones (principal-like cells) but was not affected by ATP. In this investigation, we have transfected the human Na-K-Cl cotransporter (huNKCC1) in both C11 and C7 cells to determine whether these differences in NKCC regulation by ATP were due to cell-specific purinoceptor signaling pathways or to cell-specific isoforms/splice variants of the transporter. In both cell lines, we found that endogenous as well as huNKCC1-derived cotransport activity was restricted to the basolateral side. In addition, we were able to show that extracellular application of 100 microM ATP or 100 microM UTP abolished NKCC activity in both mock- and huNKCC1-transfected C11 cells but not in mock- and huNKCC1-transfected C7 cells; in C11 cells, intriguingly, this inhibition was not affected by inhibitors of RNA and protein synthesis and occurred even though expression levels of UTP-sensitive P2Y2-, P2Y4-, and P2Y6-purinoceptors were not different from those observed in C7 cells. These results suggest that C11 cells express an undetermined type of UTP-sensitive P2-purinoceptors or a unique P2Y-purinoceptor-triggered signaling cascade that leads to inhibition of NKCC1.
Tatyana A Brindikova; Nathalie Bourcier; Brian Torres; Dimitri Pchejetski; Michel Gekle; Georgy V Maximov; Valérie Montminy; Paul A Insel; Sergei N Orlov; Paul Isenring
Related Documents :
6953128 - Ultrastructural quantitation of connective tissue changes in phenytoin-induced gingival...
18003448 - Digital holographic microscopy applied to life sciences.
1215648 - Carciac muscle cytoplasmic and nuclear development during canine neonatal growth.
2974888 - Regional changes in myocyte size during the reversal of thyroid-induced cardiac hypertr...
24084358 - Prolonged cultivation of hippocampal neural precursor cells shifts their differentiatio...
8385648 - Microinjection of intact 200- to 500-kb fragments of yac dna into mammalian cells.
Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't     Date:  2003-08-13
Journal Detail:
Title:  American journal of physiology. Cell physiology     Volume:  285     ISSN:  0363-6143     ISO Abbreviation:  Am. J. Physiol., Cell Physiol.     Publication Date:  2003 Dec 
Date Detail:
Created Date:  2003-11-05     Completed Date:  2004-01-05     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  100901225     Medline TA:  Am J Physiol Cell Physiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  C1445-53     Citation Subset:  IM    
L'Hôtel-Dieu de Québec Research Center, 10 rue McMahon (Rm. 3852 QC, Canada G1R 2J6.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Adenosine Triphosphate / metabolism,  pharmacology
Cell Line
Epithelial Cells / drug effects,  physiology
Kidney Tubules, Collecting / cytology,  physiology*
Membrane Potentials / drug effects,  physiology
Polymerase Chain Reaction
Purines / metabolism,  pharmacology
Receptors, Purinergic / metabolism
Signal Transduction / physiology*
Sodium-Potassium-Chloride Symporters / drug effects,  physiology*
Reg. No./Substance:
0/Purines; 0/Receptors, Purinergic; 0/Sodium-Potassium-Chloride Symporters; 120-73-0/purine; 56-65-5/Adenosine Triphosphate

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Calmodulin and protein kinase C regulate gap junctional coupling in lens epithelial cells.
Next Document:  Depletion of cartilage collagen fibrils in mice carrying a dominant negative Col2a1 transgene affect...