| Purine and oxypurine production in mitochondria of ischemic and reperfused myocardium. | |
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MedLine Citation:
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PMID: 2612453 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The present study was undertaken to determine whether significant breakdown of adenine nucleotides to purine bases and oxypurines occurred in mitochondria following myocardial ischemia and ischemia followed by reperfusion, and whether allopurinol prevented this effect. The adenine nucleotides adenosine, hypoxanthine, xanthine and uric acid were measured in the mitochondria and the results suggest that breakdown did occur. Malondialdehyde concentration was determined to gauge lipid peroxidation. This substance did not increase during ischemia or reperfusion, but did so in the presence of allopurinol. Xanthine dehydrogenase was converted to xanthine oxidase during reperfusion and the activity of both enzymes were inhibited by allopurinol. The results also suggested the presence of a mitochondrial 5'-nucleotidase. We conclude that significant breakdown of adenine nucleotide took place in myocardial mitochondria during ischemia and ischemia followed by reperfusion and that allopurinol may have a protective effect. |
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Authors:
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H van Jaarsveld; H C Barnard; S P Barnard; J J Maartens; G M Potgieter |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Enzyme Volume: 42 ISSN: 0013-9432 ISO Abbreviation: Enzyme Publication Date: 1989 |
Date Detail:
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Created Date: 1990-03-08 Completed Date: 1990-03-08 Revised Date: 2007-11-15 |
Medline Journal Info:
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Nlm Unique ID: 1262265 Medline TA: Enzyme Country: SWITZERLAND |
Other Details:
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Languages: eng Pagination: 136-44 Citation Subset: IM |
Affiliation:
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Department of Chemical pathology, University of the Orange Free State, Bloemfontein, Republic of South Africa. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adenine Nucleotides
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metabolism* Adenosine / metabolism Allopurinol / pharmacology Animals Hypoxanthine Hypoxanthines / metabolism Male Malondialdehyde / metabolism Mitochondria, Heart / metabolism* Myocardial Reperfusion Injury / metabolism*, prevention & control Purines / metabolism* Rats Rats, Inbred Strains Uric Acid / metabolism Xanthine Xanthine Dehydrogenase / metabolism Xanthines / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Adenine Nucleotides; 0/Hypoxanthines; 0/Purines; 0/Xanthines; 315-30-0/Allopurinol; 542-78-9/Malondialdehyde; 58-61-7/Adenosine; 68-94-0/Hypoxanthine; 69-89-6/Xanthine; 69-93-2/Uric Acid; EC 1.17.1.4/Xanthine Dehydrogenase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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