Document Detail

Purine metabolism in mesophyll protoplasts of tobacco (Nicotiana tabacum) leaves.
MedLine Citation:
PMID:  6154458     Owner:  NLM     Status:  MEDLINE    
The overall metabolism of purines was studied in tobacco (Nicotiana tabacum) mesophyll protoplasts. Metabolic pathways were studied by measuring the conversion of radioactive adenine, adenosine, hypoxanthine and guanine into purine ribonucleotides, ribonucleosides, bases and nucleic acid constituents. Adenine was extensively deaminated to hypoxanthine, whereupon it was also converted into AMP and incorporated into nucleic acids. Adenosine was mainly hydrolysed to adenine. Inosinate formed from hypoxanthine was converted into AMP and GMP, which were then catabolized to adenine and guanosine respectively. Guanine was mainly deaminated to xanthine and also incorporated into nucleic acids via GTP. Increased RNA synthesis in the protoplasts resulted in enhanced incorporation of adenine and guanine, but not of hypoxanthine and adenosine, into the nucleic acid fraction. The overall pattern of purine-nucleotide metabolic pathways in protoplasts of tobacco leaf mesophyll is proposed.
J Barankiewicz; J Paszkowski
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The Biochemical journal     Volume:  186     ISSN:  0264-6021     ISO Abbreviation:  Biochem. J.     Publication Date:  1980 Jan 
Date Detail:
Created Date:  1980-06-16     Completed Date:  1980-06-16     Revised Date:  2010-09-10    
Medline Journal Info:
Nlm Unique ID:  2984726R     Medline TA:  Biochem J     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  343-50     Citation Subset:  IM    
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MeSH Terms
Adenine / metabolism
Adenosine / metabolism
Guanine / metabolism
Hypoxanthines / metabolism
Plants / enzymology,  metabolism*
Plants, Toxic
Protoplasts / enzymology,  metabolism*
Purines / metabolism*
RNA / biosynthesis
Tobacco / enzymology,  metabolism
Reg. No./Substance:
0/Hypoxanthines; 0/Purines; 58-61-7/Adenosine; 63231-63-0/RNA; 73-24-5/Adenine; 73-40-5/Guanine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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