Document Detail

Purine bases oxidation and repair following permethrin insecticide treatment in rat heart cells.
MedLine Citation:
PMID:  20574784     Owner:  NLM     Status:  MEDLINE    
Pollutants including insecticides have been recently reported to be a risk factor involved in various diseases. Permethrin, a member of the family of synthetic pyrethroids, is widely used as insecticide in agriculture and other domestic applications. To investigate possible cardiotoxicity, we had examined different concentrations of permethrin on the freshly isolated rat heart cells using the alkaline comet assay. A significant difference in % tail DNA between all concentrations of permethrin (5, 10, 20 microM) and vehicle (control) without enzymes and with Fpg-treated cells were measured. The results indicated that permethrin induced oxidative damage to purine bases in the heart cells. Pyrimidines oxidation was evaluated using Endonuclease III (Endo III), but the results did not reveal any significant changes. After permethrin exposure, cells were studied to evaluate their DNA repair capacity. A complete DNA repair at 10 and 20 microM was measured after 30 and 60 min of repair intervals. Significant change in plasma membrane fluidity at different depths of bilayer was measured following permethrin treatment. Membrane fluidity in the hydrophilic-hydrophobic region was reduced, while the hydrophobic inner resulted more fluid following permethrin treatment of heart cells. This work points to standardize conditions applicable to ex vivo cells following in vivo treatment in order to study the cardiotoxicity of insecticide.
M S Dhivya Vadhana; Cinzia Nasuti; Rosita Gabbianelli
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cardiovascular toxicology     Volume:  10     ISSN:  1559-0259     ISO Abbreviation:  Cardiovasc. Toxicol.     Publication Date:  2010 Sep 
Date Detail:
Created Date:  2010-08-24     Completed Date:  2010-12-08     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101135818     Medline TA:  Cardiovasc Toxicol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  199-207     Citation Subset:  IM    
School of Advanced Studies, UNICAM, Camerino, Italy.
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MeSH Terms
Biological Markers
Cell Count
Cell Separation
Cell Survival / drug effects
Comet Assay
DNA Damage
DNA Repair / drug effects*
Heart / drug effects*
Insecticides / toxicity*
Membrane Fluidity / drug effects
Myocardium / metabolism,  pathology
Permethrin / toxicity*
Purines / metabolism*
Reg. No./Substance:
0/Biological Markers; 0/Insecticides; 0/Purines; 52645-53-1/Permethrin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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