Document Detail


Purified human early pregnancy factor from preimplantation embryo possesses immunosuppresive properties.
MedLine Citation:
PMID:  2712125     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
This study was undertaken to determine whether early pregnancy factor secreted by preimplantation embryos has immunosuppressive properties. Human early pregnancy factor was purified from embryo growth media of in vitro fertilized ova with ion-exchange and gel filtration chromatography. During each step of purification the fractions were tested for (1) early pregnancy factor activity with the rosette inhibition assay, (2) immunosuppressive properties with a concanavalin A-stimulated lymphocyte proliferation assay, and (3) purity by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Results indicate that (1) human early pregnancy factor has a basic molecular weight of 14 kd, (2) early pregnancy factor has immunosuppressive activity, (3) polymers of early pregnancy factor also appear to be present in the embryo growth media, and (4) immunosuppressive factors other than early pregnancy factor are also secreted by preimplantation human embryos. Early pregnancy factor and other factor(s) produced by the preimplantation embryo may play a role in suppressing maternal cellular immune responses, thereby preventing maternal rejection of the embryo.
Authors:
R Bose; H Cheng; E Sabbadini; J McCoshen; M M MaHadevan; J Fleetham
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  American journal of obstetrics and gynecology     Volume:  160     ISSN:  0002-9378     ISO Abbreviation:  Am. J. Obstet. Gynecol.     Publication Date:  1989 Apr 
Date Detail:
Created Date:  1989-05-31     Completed Date:  1989-05-31     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0370476     Medline TA:  Am J Obstet Gynecol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  954-60     Citation Subset:  AIM; IM    
Affiliation:
Department of Obstetrics, Gynecology and Reproductive Sciences, University of Manitoba, Winnipeg, Canada.
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MeSH Terms
Descriptor/Qualifier:
Blastocyst / analysis*
Carboxymethylcellulose / diagnostic use
Cell Division / drug effects
Chaperonin 10
Chromatography / methods
Chromatography, DEAE-Cellulose
Chromatography, Gel
Concanavalin A / pharmacology
Female
Humans
Immunosuppressive Agents*
Lymphocytes / cytology
Molecular Weight
Peptides / analysis,  isolation & purification*,  pharmacology
Pregnancy
Pregnancy Proteins*
Suppressor Factors, Immunologic*
Chemical
Reg. No./Substance:
0/Chaperonin 10; 0/Immunosuppressive Agents; 0/Peptides; 0/Pregnancy Proteins; 0/Suppressor Factors, Immunologic; 0/early pregnancy factor; 11028-71-0/Concanavalin A; 9004-32-4/Carboxymethylcellulose

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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