| Purification of two cytochrome P450 isozymes related to CYP2A and CYP3A gene families from monkey (baboon, Papio papio) liver microsomes. Cross reactivity with human forms. | |
| | |
MedLine Citation:
|
PMID: 1541278 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Two cytochrome P450 isozymes, FA and FI, were isolated and characterized from liver microsomes of phenobarbital-induced baboons (Papio papio). The cytochrome FA possesses the same N-terminal amino acid sequence as P450 MK2 from crab-eating monkeys (Macaca irus) and closely resembles the human P450 3A isozymes. This cytochrome was able to oxidize nifedipine and hydroxylate testosterone at the 6 beta position. The second baboon cytochrome (FI) is closely related to the P450 2A subfamily and has the same N-terminal sequence as human P450 2A7. Like human P450 2A forms, it is highly active as a coumarin 7-hydroxylase. Antibodies against P450 FA and FI cross-react with two human liver proteins of 51 kDa and 49 kDa, respectively. The concentration of the first protein in the human samples, was five-times greater than the second. However, the latter showed marked interindividual variation. In primary cultures of human hepatocytes, rifampicin is a strong inducer of the 51-kDa protein and a moderate inducer of the 49-kDa protein, while phenobarbital has the opposite effect on the two proteins. |
| | |
Authors:
|
I Dalet-Beluche; X Boulenc; G Fabre; P Maurel; C Bonfils |
Related Documents
:
|
8316088 - Virulence of yersinia enterocolitica is closely associated with siderophore production,... 16787928 - Rna silencing of mitochondrial m-nfs1 reduces fe-s enzyme activity both in mitochondria... 9539148 - Papain proteolysis releases a soluble nadph dependent diaphorase activity from bovine n... 3182798 - The site of synthesis of the iron-sulfur subunits of the flavoprotein and iron-protein ... 1827228 - Isolation and characterization of insertion mutants in e1a of adenovirus type 5. 14729708 - The trans-acting protein interacting with the dna motif proximal to the transcriptional... |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: European journal of biochemistry / FEBS Volume: 204 ISSN: 0014-2956 ISO Abbreviation: Eur. J. Biochem. Publication Date: 1992 Mar |
Date Detail:
|
Created Date: 1992-04-07 Completed Date: 1992-04-07 Revised Date: 2007-07-23 |
Medline Journal Info:
|
Nlm Unique ID: 0107600 Medline TA: Eur J Biochem Country: GERMANY |
Other Details:
|
Languages: eng Pagination: 641-8 Citation Subset: IM |
Affiliation:
|
Institut National de la Santé et de la Recherche Médicale, Unité 128, Montpellier, France. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Amino Acid Sequence Animals Aryl Hydrocarbon Hydroxylases* Blotting, Western Cross Reactions Cytochrome P-450 Enzyme System / biosynthesis, genetics, isolation & purification*, metabolism Electrophoresis, Polyacrylamide Gel Enzyme Induction Humans Hydroxylation Isoenzymes / biosynthesis, genetics, isolation & purification*, metabolism Male Microsomes, Liver / drug effects, enzymology* Mixed Function Oxygenases / metabolism Molecular Sequence Data Multigene Family* Papio Phenobarbital / pharmacology Sequence Homology, Nucleic Acid Testosterone / metabolism Xenobiotics / metabolism |
| Chemical | |
Reg. No./Substance:
|
0/Isoenzymes; 0/Xenobiotics; 50-06-6/Phenobarbital; 58-22-0/Testosterone; 9035-51-2/Cytochrome P-450 Enzyme System; EC 1.-/Mixed Function Oxygenases; EC 1.14.14.1/Aryl Hydrocarbon Hydroxylases; EC 1.14.14.1/CYP3A protein, human; EC 1.14.14.1/coumarin 7-hydroxylase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Phenylalanine ammonia-lyase in potato (Solanum tuberosum L.). Genomic complexity, structural compari...
Next Document: Stabilization of recombinant human basic fibroblast growth factor by chemical modifications of cyste...