| Purification of three pectin esterases from ripe peach fruit. | |
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MedLine Citation:
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PMID: 7765664 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Three isoforms of pectin esterase (PE1-PE3) (pectin pectyl-hydrolase, EC 3.1.1.11.) were purified to homogeneity from ripe peach fruit (Prunus persica cv. Coronet). The three enzymes were basic proteins of M(r) 34,000 as determined by denaturing polyacrylamide gel electrophoresis but were separated by FPLC cation-exchange chromatography. The proteins were N-terminally blocked but amino acid sequences were obtained for peptides released from two of the three isoforms. The sequences revealed a threonine/lysine substitution in a comparison between isoform PE2/isoform PE3, and there were regions of sequence similarity with other plant pectin esterases. The proteins did not bind to concanavalin A and were not stained by the periodate-Schiff reagent suggesting a low or zero level of glycosylation. Polyclonal antisera to isoform PE3 also bound to isoforms PE1 and PE2. The study provides the enzyme protein sequence and immunological basis for an evaluation of the role of pectin esterases in normal and abnormal ripening of peach fruit. |
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Authors:
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H Glover; C Brady |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Phytochemistry Volume: 37 ISSN: 0031-9422 ISO Abbreviation: Phytochemistry Publication Date: 1994 Nov |
Date Detail:
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Created Date: 1995-01-30 Completed Date: 1995-01-30 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0151434 Medline TA: Phytochemistry Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 949-55 Citation Subset: B |
Affiliation:
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CSIRO Division of Horticulture and Macquarie University, North Ryde, New South Wales, Australia. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Amino Acids / analysis Carboxylic Ester Hydrolases / isolation & purification*, metabolism Fruit / enzymology* Isoenzymes / isolation & purification*, metabolism Molecular Sequence Data Sequence Homology, Amino Acid |
| Chemical | |
Reg. No./Substance:
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0/Amino Acids; 0/Isoenzymes; EC 3.1.1.-/Carboxylic Ester Hydrolases; EC 3.1.1.11/pectinesterase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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