Document Detail

Purification and structure analysis of mycolic acids in Corynebacterium glutamicum.
MedLine Citation:
PMID:  22538651     Owner:  NLM     Status:  In-Data-Review    
Corynebacterium glutamicum is widely used for producing amino acids. Mycolic acids, the major components in the cell wall of C. glutamicum might be closely related to the secretion of amino acids. In this study, mycolic acids were extracted from 5 strains of C. glutamicum, including ATCC 13032, ATCC 13869, ATCC 14067, L-isoleucine producing strain IWJ-1, and L-valine producing strain VWJ-1. Structures of these mycolic acids were analyzed using thin layer chromatography and electrospray ionization mass spectrometry. More than twenty molecular species of mycolic acid were observed in all 5 strains. They differ in the length (20-40 carbons) and saturation (0-3 double bonds) of their constituent fatty acids. The dominant species of mycolic acid in every strain was different, but their two hydrocarbon chains were similar in length (14-18 carbons), and the meromycolate chain usually contained double bonds. As the growth temperature of cells increased from 30°C to 34°C, the proportion of mycolic acid species containing unsaturated and shorter hydrocarbon chains increased. These results provide new information on mycolic acids in C. glutamicum, and could be useful for modifying the cell wall to increase the production of amino acids.
Yang Yang; Feng Shi; Guanjun Tao; Xiaoyuan Wang
Publication Detail:
Type:  Journal Article     Date:  2012-04-27
Journal Detail:
Title:  Journal of microbiology (Seoul, Korea)     Volume:  50     ISSN:  1976-3794     ISO Abbreviation:  J. Microbiol.     Publication Date:  2012 Apr 
Date Detail:
Created Date:  2012-04-27     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9703165     Medline TA:  J Microbiol     Country:  Korea (South)    
Other Details:
Languages:  eng     Pagination:  235-40     Citation Subset:  IM    
State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, 214122, P. R. China.
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