Document Detail


Purification and structural characterization of de-N-acetylated form of GD3 ganglioside present in human melanoma tumors.
MedLine Citation:
PMID:  17242043     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The presence of gangliosides containing de-N-acetylated sialic acids in human tissues has been so far shown by using mouse monoclonal antibodies specific for the de-N-acetylated forms, but the isolation and chemical characterization of such compounds have not yet been performed. Since indirect evidence suggested that de-N-acetylGD3 ganglioside could be present in human melanoma tumors, we analyzed the gangliosides purified from a 500-g pool of those tumors. The de-N-acetylGD3 that was found to migrate just below GD2 in thin-layer chromatography was isolated from the disialogangliosides by high-pressure liquid chromatography using the specific antibody SGR37 to monitor the elution. The amount of antigen was found to be 320 ng per gram of fresh tumor or 0.1% of total gangliosides. Gas chromatography-mass spectrometry analysis of the antibody-positive ganglioside showed that sialic acids were formed of one molecule of N-acetylneuraminic acid and one molecule of neuraminic acid. Radioactive re-N-acetylation of the antigen yielded a GD3-like ganglioside with the radioactive label on the external sialic acid. The constitutive fatty acids were found to differ markedly from those of GD3 and 9-O-acetylGD3 isolated from the same pool of tumors. The major fatty acids were C16:0 and C18:0 in de-N-acetylGD3, whereas GD3 and its 9-O-acetylated derivative contained a large amount of C24:1. These data show that de-N-acetylGD3 ganglioside is indeed present in human melanoma tumors, and the fatty acid content suggests the existence of a de-N-acetylase mostly active on the molecular species of gangliosides with short-chain fatty acids.
Authors:
Iuliana Popa; Alexandre Pons; Christophe Mariller; Tadashi Tai; Jean-Pierre Zanetta; Luc Thomas; Jacques Portoukalian
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Publication Detail:
Type:  Journal Article     Date:  2007-01-22
Journal Detail:
Title:  Glycobiology     Volume:  17     ISSN:  0959-6658     ISO Abbreviation:  Glycobiology     Publication Date:  2007 Apr 
Date Detail:
Created Date:  2007-03-15     Completed Date:  2007-06-26     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9104124     Medline TA:  Glycobiology     Country:  England    
Other Details:
Languages:  eng     Pagination:  367-73     Citation Subset:  IM    
Affiliation:
Laboratory of Dermatological Research, University of Lyon-1 and Edouard Herriot Hospital, 69437 Lyon Cx 03, France.
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MeSH Terms
Descriptor/Qualifier:
Acetylation
Carbohydrate Conformation
Gangliosides / chemistry*,  isolation & purification*
Humans
Immunohistochemistry
Melanoma / metabolism*,  pathology,  surgery
Models, Molecular
N-Acetylneuraminic Acid / isolation & purification
Chemical
Reg. No./Substance:
0/Gangliosides; 131-48-6/N-Acetylneuraminic Acid; 62010-37-1/ganglioside, GD3

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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