Document Detail


Purification and structural characterisation of lipid transfer protein from red wine and grapes.
MedLine Citation:
PMID:  23265486     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Lipid transfer proteins (LTP) play a major role in plant defence and are of particular interest due to their known ability to cause allergic reactions. These proteins are expressed in grapes and also remain detectable after vinification, especially in red wine. However, it remains unknown whether the protein undergoes any changes during the vinification process. Here, we present a purification method for LTPs from Dornfelder grapes and wine. By liquid-chromatography-mass spectroscopy (LC-MS/MS) we identified LTPs from two different species (Vitis vinifera and Vitis aestivalis). Additionally, the purified LTPs were characterised using spectrometric methods, confirming their high purity and structural stability during vinification. We conclude that LTPs are resistant to the alcohol content (13.5vol%), acidic milieu of wine and other ingredients present during the vinification process, indicating that the allergenic potential of grape LTP is not diminished by the vinification process.
Authors:
Nadine Jaeckels; Stefan Tenzer; Susanne Rosfa; Hansjoerg Schild; Heinz Decker; Petra Wigand
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Publication Detail:
Type:  Journal Article     Date:  2012-11-08
Journal Detail:
Title:  Food chemistry     Volume:  138     ISSN:  0308-8146     ISO Abbreviation:  Food Chem     Publication Date:  2013 May 
Date Detail:
Created Date:  2012-12-25     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7702639     Medline TA:  Food Chem     Country:  England    
Other Details:
Languages:  eng     Pagination:  263-9     Citation Subset:  IM    
Copyright Information:
Copyright © 2012 Elsevier Ltd. All rights reserved.
Affiliation:
Institute for Molecular Biophysics, Johannes Gutenberg-University Mainz, Jakob Welder-Weg 26, 55128 Mainz, Germany.
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