| Purification of streptodornase from Streptococcus equisimilis and its DNA-induced conformational change. | |
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MedLine Citation:
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PMID: 9020028 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Extracellular streptodornase was purified from fermentation media of Streptococcus equisimilis by stepwise carboxymethyl-Sepharose column chromatography. The active enzyme fraction was eluted with phosphate buffer containing 0.2 M NaCl. The purified enzyme showed a homogeneity on SDS-PAGE and had a subunit molecular weight of 35 kDa. Conformational change of streptodornase by binding to calf thymus DNA was examined by circular dichroism (CD). CD study clearly showed a DNA-induced conformational change in the secondary structure of streptodornase, resulting in a decrease of alpha-helical content of the enzyme. |
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Authors:
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M Lee; K B Song |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Biochemical and biophysical research communications Volume: 230 ISSN: 0006-291X ISO Abbreviation: Biochem. Biophys. Res. Commun. Publication Date: 1997 Jan |
Date Detail:
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Created Date: 1997-02-13 Completed Date: 1997-02-13 Revised Date: 2004-11-17 |
Medline Journal Info:
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Nlm Unique ID: 0372516 Medline TA: Biochem Biophys Res Commun Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 13-5 Citation Subset: IM |
Affiliation:
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Department of Food Science and Technology, College of Agriculture, Chungnam National University, Taejon, Korea. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cattle Chromatography, Ion Exchange Circular Dichroism DNA / chemistry*, metabolism Deoxyribonuclease I / chemistry*, isolation & purification*, metabolism Electrophoresis, Polyacrylamide Gel Macromolecular Substances Molecular Weight Protein Binding Protein Conformation* Streptococcus / enzymology* |
| Chemical | |
Reg. No./Substance:
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0/Macromolecular Substances; 9007-49-2/DNA; EC 3.1.21.1/Deoxyribonuclease I |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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