| Purification and stability characterization of a cell regulatory sialoglycopeptide inhibitor. | |
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MedLine Citation:
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PMID: 8530539 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Previous attempts to physically separate the cell cycle inhibitory and protease activities in preparations of a purified cell regulatory sialoglycopeptide (CeReS) inhibitor were largely unsuccessful. Gradient elution of the inhibitor preparation from a DEAE HPLC column separated the cell growth inhibitor from the protease, and the two activities have been shown to be distinct and non-overlapping. The additional purification increased the specific biological activity of the CeReS preparation by approximately two-fold. The major inhibitory fraction that eluted from the DEAE column was further analyzed by tricine-SDS-PAGE and microbore reverse phase HPLC and shown to be homogeneous in nature. Two other fractions separated by DEAE HPLC, also devoid of protease activity, were shown to be inhibitory to cell proliferation and most likely represented modified relatives of the CeReS inhibitor. The highly purified CeReS was chemically characterized for amino acid and carbohydrate composition and the role of the carbohydrate in cell proliferation inhibition, stability, and protease resistance was assessed. |
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Authors:
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P J Moos; H K Fattaey; T C Johnson |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Journal of cellular biochemistry Volume: 59 ISSN: 0730-2312 ISO Abbreviation: J. Cell. Biochem. Publication Date: 1995 Sep |
Date Detail:
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Created Date: 1996-01-30 Completed Date: 1996-01-30 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 8205768 Medline TA: J Cell Biochem Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 79-90 Citation Subset: IM; S |
Affiliation:
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Center for Basic Cancer Research, Kansas State University, Manhattan 66506-4903, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acids
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analysis Animals Carbohydrates / analysis Cattle Cell Division / drug effects Cells, Cultured Chromatography, Gel Chromatography, High Pressure Liquid Chromatography, Ion Exchange Electrophoresis, Polyacrylamide Gel Hot Temperature Hydrolysis Sialoglycoproteins / chemistry, isolation & purification*, metabolism, pharmacology |
| Grant Support | |
ID/Acronym/Agency:
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CA09418/CA/NCI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Amino Acids; 0/Carbohydrates; 0/Sialoglycoproteins |
| Investigator | |
Investigator/Affiliation:
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B S Spooner / KS St U, Manhattan |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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